Rabbit anti-Human PRMT4 Polyclonal Antibody | anti-CARM1 antibody

PRMT4 (CARM1, Protein arginine N Methyltransferase 4)

Cat. Num. AAA611140

• Gene Names: CARM1; PRMT4
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PRMT4; Polyclonal Antibody; PRMT4 (CARM1; Protein arginine N Methyltransferase 4); Anti -PRMT4 (CARM1; anti-CARM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CARM1 (PRMT4).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide and 0.1mg/ml BSA.

• Applicable Applications for anti-CARM1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-2ug/ml
• Immunogen: Synthetic peptides correspnding to aa45-69 and 595-608 of human PRMT4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRMT4 in cell lysates from HeLa cells using MBS611140 at 0.5 ug /ml. 20ug of HeLa cell lysate was loaded per well of mini gel.)

Related Product Information for anti-CARM1 antibody

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. CARM1 (coactivator-associated arginine methyltransferase-1) also known as PRMT4 (protein-arginine N-methyltransferase-4) belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain-2 of the SRC (steroid receptor coactivator) GRIP-1, whereas p300 interacts with activation domain-1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Product Categories/Family for anti-CARM1 antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 530414438
• NCBI GeneID: 10498
• NCBI Accession #: XP_005259765.1
• NCBI GenBank Nucleotide #: XM_005259708.1
• UniProt Accession #: Q86X55; A6NN38
• Molecular Weight: 65,854 Da
• NCBI Official Full Name: histone-arginine methyltransferase CARM1 isoform X1
• NCBI Official Synonym Full Names: coactivator-associated arginine methyltransferase 1
• NCBI Official Symbol: CARM1
• NCBI Official Synonym Symbols: PRMT4
• NCBI Protein Information: histone-arginine methyltransferase CARM1; protein arginine N-methyltransferase 4
• UniProt Protein Name: Histone-arginine methyltransferase CARM1
• Protein Family: Probable histone-arginine methyltransferase
• UniProt Gene Name: CARM1
• UniProt Synonym Gene Names: PRMT4
• UniProt Entry Name: CARM1_HUMAN

NCBI Description

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]

Uniprot Description

CARM1: a protein arginine methyltransferase that methylates histones and other proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginine residues. A key epigenetic regulator of hematopoiesis affecting multiple lineages. Recruited to promoters upon gene activation together with histone acetyltransferases from P300 and p160 families, methylates histone H3 at R17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2 activation, acts synergically with P300 and either one of the p160 histone acetyltransferases SRC1, NCoA2 and SRC-3 or CTNNB1 to activate transcription. During myogenic transcriptional activation, acts together with SRC-3 as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53 transcriptional activation. Methylates P300, both at R 2142, which may loosen its interaction with NCoA2, and at R580 and R604 in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Methylates RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. Interacts with the C-terminus of NCoA2, SRC-3 and SRC1. Part of a complex consisting of CARM1, P300 and NCoA2. Interacts with FLII, TP53, myogenic factor MEF2, P300, TRIM24, CREBBP and CTNNB1. Identified in a complex containing CARM1, TRIM24 and NCoA2. Interacts with SRC3. Interacts with SNRPC. Interacts with NR1H4. Interacts with RELA. Interacts with HTLV-1 Tax-1. Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 K18 (H3K18ac) H3 K23 (H3K23ac) by EP300 and blocked by citrullination of H3 R17 (H3R17ci) by PADI4. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.125; Methyltransferase; Nuclear receptor co-regulator; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; protein-arginine N-methyltransferase activity; protein binding; protein homodimerization activity; ligand-dependent nuclear receptor transcription coactivator activity; transcription coactivator activity; histone-arginine N-methyltransferase activity; beta-catenin binding; protein methyltransferase activity

Biological Process: histone methylation; regulation of estrogen receptor signaling pathway; establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; response to cAMP; viral reproduction; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of fat cell differentiation; pathogenesis; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; cellular lipid metabolic process; negative regulation of protein binding