Goat PRMT3 Polyclonal Antibody | anti-PRMT3 antibody

Goat anti-PRMT3 (aa468-421) Antibody

Cat. Num. AAA423160

• Gene Names: PRMT3; HRMT1L3
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PRMT3; Polyclonal Antibody; Goat anti-PRMT3 (aa468-421) Antibody; protein arginine methyltransferase 3; HRMT1L3; HMT1 hnRNP methyltransferase-like 3; OTTHUMP00000231732; OTTHUMP00000231735; heterogeneous nuclear ribonucleoprotein methyltransferase-like 3; heterogeneous nuclear ribonucleoprotein methyltransferase-; heterogeneous nuclear ribonucleoprotein methyltransferase-like 3 antibody; heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3 antibody; HMT1 hnRNP methyltransferase-like 3 antibody; HRMT1L3 antibody; OTTHUMP00000231732 antibody; OTTHUMP00000231735 antibody; PRMT3 antibody; protein arginine methyltransferase 3 antibody; protein arginine N-methyltransferase 3 antibody; PRMT3 (aa468-421); anti-PRMT3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DPKTLISEPCGIKH
• Sequence Length: 531

• Applicable Applications for anti-PRMT3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx 70kDa band observed in lysates of cell line MCF7 (calculated MW of 59.9kDa according to NP_005779.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Swiercz et al, J Biol Chem.2007 Jun 8;282(23): 16917-23. PMID: 17439947). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence DPKTLISEPCGIKH, from the internal region of the protein sequence according to NP_005779.1; NP_001138638.1; NP_001138639.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 44771198
• NCBI GeneID: 10196
• NCBI Accession #: NP_005779.1; NP_001138638.1; NP_001138639.1
• NCBI GenBank Nucleotide #: NM_005788.3
• Molecular Weight: 52,875 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 3 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 3
• NCBI Official Symbol: PRMT3
• NCBI Official Synonym Symbols: HRMT1L3
• NCBI Protein Information: protein arginine N-methyltransferase 3
• UniProt Protein Name: Protein arginine N-methyltransferase 3
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT3
• UniProt Synonym Gene Names: HRMT1L3
• UniProt Entry Name: ANM3_HUMAN

NCBI Description

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

PRMT3: Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins. Belongs to the protein arginine N-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein arginine; Methyltransferase; EC 2.1.1.-; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; cytosol; ribosome

Molecular Function: histone-arginine N-methyltransferase activity; metal ion binding; methyltransferase activity; protein binding; protein-arginine N-methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity

Biological Process: negative regulation of protein ubiquitination; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; regulation of transcription, DNA-dependent