Western Blot (WB)
(HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with (0.1ug/ml) in top panel, probed with)
Goat PRKD1 Polyclonal Antibody | anti-PRKD1 antibody
Goat Anti-PRKD1 (aa233-246) Antibody
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig
Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig
Western Blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommended concentration 0.1-0.3ug/ml
Peptide ELISA: Antibody detection limit dilution 1:128000.
Western Blot (WB)
(HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with (0.1ug/ml) in top panel, probed with)
Western Blot (WB)
(HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with (0.1ug/ml) in top panel, probed with)
Western Blot (WB)
(HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug The precipitate was subsequently probed in Western blot using at 1ug/ml. The secondary anti-goat picks up the heavy chain of used for the immunoprecipitation (annotated as IgG). Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA)
Western Blot (WB)
(HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug The precipitate was subsequently probed in Western blot using at 1ug/ml. The secondary anti-goat picks up the heavy chain of used for the immunoprecipitation (annotated as IgG). Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA)
NCBI and Uniprot Product Information
NCBI Description
PRKD1 is a serine/threonine kinase that regulates a variety of cellular functions, including membrane receptor signaling, transport at the Golgi, protection from oxidative stress at the mitochondria, gene transcription, and regulation of cell shape, motility, and adhesion (summary by Eiseler et al., 2009 [PubMed 19329994]).[supplied by OMIM, Nov 2010]
Uniprot Description
PRKD1: a CAMK kinase of the PKD family. Cleavage by caspase-3 following DNA damage activates it and alters its subcellular localization. Sensitizes cells to apoptosis induced by genotoxic stress. Its cleavage is blocked in cells that over-express the anti-apoptotic Bcl-x(L) protein. Expression of a caspase-resistant mutant partially inhibits DNA damage-induced apoptosis. Its activation by TLR ligands is dependent on MyD88, IRAK4 and -1, but not TRAF6. Essential for MyD88-dependent proinflammatory immune responses. Activated by diacylglycerol and phorbol esters. Binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface. Colocalizes with F-actin at peripheral F-actin-rich structures in membrane ruffles at the edge of lamellipodia in cervical carcinoma cells. Substrates reportedly include critical regulatory proteins including CREB, SSH1L, CTNNB1, HDACs 5 and 7, PKD1, HPK1, MARK2, PIP5K2A, PPP1R14A
Protein type: Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Autophagy; Kinase, protein; EC 2.7.11.13; CAMK group; PKD family
Chromosomal Location of Human Ortholog: 14q11
Cellular Component: cytoplasm; cytosol; Golgi apparatus; integral to plasma membrane; plasma membrane; trans-Golgi network
Molecular Function: identical protein binding; kinase activity; protein binding; protein kinase C activity; protein serine/threonine kinase activity
Biological Process: activation of CREB transcription factor; activation of NF-kappaB transcription factor; cell proliferation; Golgi organization and biogenesis; Golgi vesicle transport; integrin-mediated signaling pathway; negative regulation of endocytosis; peptidyl-serine phosphorylation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; protein amino acid phosphorylation; Ras protein signal transduction; regulation of protein stability; signal transduction; sphingolipid biosynthetic process; vascular endothelial growth factor receptor signaling pathway
Research Articles on PRKD1
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Product Notes
The PRKD1 prkd1 (Catalog #AAA423679) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-PRKD1 (aa233-246) Antibody reacts with Human, Mouse, Rat, Pig Tested: Human Expected from sequence similarity: Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's PRKD1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), WB-Trf, Immunoprecipitation (IP). Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody. Western Blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommended concentration 0.1-0.3ug/ml Peptide ELISA: Antibody detection limit dilution 1:128000. Researchers should empirically determine the suitability of the PRKD1 prkd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QKSPSESFIG REKR. It is sometimes possible for the material contained within the vial of "PRKD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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