Rabbit PRKCH Polyclonal Antibody | anti-PRKCH antibody

PRKCH Antibody

Cat. Num. AAA9434834

• Gene Names: PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: PRKCH; Polyclonal Antibody; PRKCH Antibody; PKCL; PKC-L; PRKCL; nPKC-eta; anti-PRKCH antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Antigen affinity purification
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 0.8mg/mL (varies by lot)

• Applicable Applications for anti-PRKCH antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:100
• Immunogen: Synthetic peptide of human PRKCH
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C for 1 year.

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human colorectal cancer using (PRKCH Antibody) at dilution 1/35.)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human gastric cancer using (PRKCH Antibody) at dilution 1/35.)

Related Product Information for anti-PRKCH antibody

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Product Categories/Family for anti-PRKCH antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 28557781
• NCBI GeneID: 5583
• NCBI Accession #: NP_006246
• NCBI GenBank Nucleotide #: NM_006255.4
• UniProt Accession #: O75541; Q16246; Q8NE03; B4DJN5; P24723
• Molecular Weight: 59,521 Da
• NCBI Official Full Name: protein kinase C eta type
• NCBI Official Synonym Full Names: protein kinase C eta
• NCBI Official Symbol: PRKCH
• NCBI Official Synonym Symbols: PKCL; PKC-L; PRKCL; nPKC-eta
• NCBI Protein Information: protein kinase C eta type
• UniProt Protein Name: Zinc finger protein 821
• Protein Family: Protein kinase
• UniProt Gene Name: ZNF821
• UniProt Entry Name: ZN821_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]

Uniprot Description

ZNF821: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent