Rabbit anti-Human PRKCH Polyclonal Antibody | anti-PRKCH antibody

Anti-PRKCH Antibody

Cat. Num. AAA415562

• Gene Names: PRKCH; PKCL; PKC-L; PRKCL; nPKC-eta
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: PRKCH; Polyclonal Antibody; Anti-PRKCH Antibody; anti-PRKCH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 3522

• Applicable Applications for anti-PRKCH antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA titer: 1:1,000-1:10,000; Western blot analysis: 1:200-500; Optimal dilutions/concentrations should be determined by the end user
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-PRKCH antibody

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. This gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

References

(1) Teixeira,V.H., Jacq,L., Moore,J., et,al. Association and expression study of PRKCH gene in a French Caucasian population with rheumatoid arthritis. J. Clin. Immunol. 2008;28 (2):115-121.

NCBI and Uniprot Product Information

• NCBI GI #: 47717140
• NCBI GeneID: 5583
• NCBI Accession #: NP_006246.2
• NCBI GenBank Nucleotide #: NM_006255
• Molecular Weight: 59,521 Da
• NCBI Official Full Name: Homo sapiens protein kinase C, eta (PRKCH), mRNA
• NCBI Official Synonym Full Names: protein kinase C, eta
• NCBI Official Symbol: PRKCH
• NCBI Official Synonym Symbols: PKCL; PKC-L; PRKCL; nPKC-eta
• NCBI Protein Information: protein kinase C eta type
• UniProt Protein Name: Protein kinase C eta type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCH
• UniProt Entry Name: KPCL_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCH: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-p27Kip1 complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1- dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Interacts with FYN and RALA. Interacts with DGKQ. Most abundant in lung, less in heart and skin. Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; AGC group; PKC family; Eta subfamily

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: calcium-independent protein kinase C activity; protein kinase C activity; enzyme binding; metal ion binding; Ral GTPase binding; ATP binding

Biological Process: platelet activation; positive regulation of B cell receptor signaling pathway; signal transduction; blood coagulation; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of keratinocyte differentiation

Disease: Stroke, Ischemic