Rabbit PRKAR2A Polyclonal Antibody | anti-PRKAR2A antibody

PRKAR2A (cAMP-dependent Protein Kinase Type II-alpha Regulatory Subunit, MGC3606, PKR2, PRKAR2) (PE)

Cat. Num. AAA6432930

• Gene Names: PRKAR2A; PKR2; PRKAR2
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PRKAR2A; Polyclonal Antibody; PRKAR2A (cAMP-dependent Protein Kinase Type II-alpha Regulatory Subunit; MGC3606; PKR2; PRKAR2) (PE); anti-PRKAR2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PRKAR2A. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PRKAR2A antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1:20-1:50; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PRKAR2A.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cell and Hela cell line, using PRKAR2A antibody.)

Product Categories/Family for anti-PRKAR2A antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4758958
• NCBI GeneID: 5576
• NCBI Accession #: NP_004148.1
• NCBI GenBank Nucleotide #: NM_004157.2
• UniProt Accession #: P13861; Q16823
• Molecular Weight: 45,518 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, alpha
• NCBI Official Symbol: PRKAR2A
• NCBI Official Synonym Symbols: PKR2; PRKAR2
• NCBI Protein Information: cAMP-dependent protein kinase type II-alpha regulatory subunit; protein kinase A, RII-alpha subunit; cAMP-dependent protein kinase regulatory subunit RII alpha
• UniProt Protein Name: cAMP-dependent protein kinase type II-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR2A
• UniProt Synonym Gene Names: PKR2; PRKAR2
• UniProt Entry Name: KAP2_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 3p21.3-p21.2

Cellular Component: centrosome; focal adhesion; membrane; cytoplasm; plasma membrane; cytosol; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex

Molecular Function: protein binding; cAMP-dependent protein kinase inhibitor activity; ubiquitin protein ligase binding; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; phospholipase C activation; water transport; activation of protein kinase A; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; signal transduction; regulation of insulin secretion; transmembrane transport