Rabbit PRKAR1A Polyclonal Antibody | anti-PRKAR1A antibody

PRKAR1A Rabbit Polyclonal

Cat. Num. AAA7603012

• Gene Names: PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PRKAR1A; Polyclonal Antibody; PRKAR1A Rabbit Polyclonal; anti-PRKAR1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 381

• Applicable Applications for anti-PRKAR1A antibody: ELISA (EIA), Western Blot (WB)
• Immunogen: Protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human oophoroma using MBS7603012 (PRKAR1A antibody) at dilution of 1:100)

Western Blot (WB)

(HeLa cells were subjected to SDS PAGE followed by western blot with MBS7603012 (PRKAR1A antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 823669506
• NCBI GeneID: 5573
• Molecular Weight: 38,003 Da
• NCBI Official Full Name: PRKAR1A, partial
• NCBI Official Synonym Full Names: protein kinase cAMP-dependent type I regulatory subunit alpha
• NCBI Official Symbol: PRKAR1A
• NCBI Official Synonym Symbols: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR1A
• UniProt Synonym Gene Names: PKR1; PRKAR1; TSE1; TSE1
• UniProt Entry Name: KAP0_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed. [provided by RefSeq, Jan 2013]

Uniprot Description

PKAR1A: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Interacts with RFC2: the complex may be involved in cell survival. Defects in PRKAR1A are the cause of Carney complex type 1 (CNC1). CNC is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumors, and psammomatous melanotic schwannomas.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: AMP-activated protein kinase complex; cAMP-dependent protein kinase complex; cytoplasm; cytosol; immunological synapse; membrane; neuromuscular junction; protein complex

Molecular Function: cAMP binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; protein binding; ubiquitin protein ligase binding

Biological Process: activation of protein kinase A; blood coagulation; cardiac muscle cell proliferation; female meiosis; mesoderm formation; negative regulation of activated T cell proliferation; negative regulation of meiosis; regulation of transcription from RNA polymerase II promoter; renal water homeostasis; sarcomere organization

Disease: Acrodysostosis 1 With Or Without Hormone Resistance; Carney Complex, Type 1; Myxoma, Intracardiac; Pigmented Nodular Adrenocortical Disease, Primary, 1; Thyroid Carcinoma, Papillary