Goat anti-Human PRK / PLK3 Polyclonal Antibody | anti-PRK antibody

Goat Polyclonal to Human PRK / PLK3

Cat. Num. AAA248481

• Gene Names: PLK3; CNK; FNK; PRK
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PRK / PLK3; Polyclonal Antibody; Goat Polyclonal to Human PRK / PLK3; Anti-PRK / PLK3 Antibody (aa320-333) IHC-plus; PLK3; CNK; FNK; Polo-like kinase 3; PRK; Proliferation-related kinase; Cytokine-inducible kinase; FGF-inducible kinase; PLK-3; Human PRK; anti-PRK antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human PRK / PLK3
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 646

• Applicable Applications for anti-PRK antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line HeLa (calculated MW of 71.6kD according to NP_004064.2). Recommended concentration: 1-3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide PDRLPISSCVTVPD from an internal region of human PRK / PLK3 (NP_004064.2). Percent identity by BLAST analysis: Hamster (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: PRK / PLK3 antibody was raised against synthetic peptide PDRLPISSCVTVPD from an internal region of human PRK / PLK3 (NP_004064.2). Percent identity by BLAST analysis: Hamster (86%).
• Antigen Modification: aa320-333
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PRK / PLK3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Huma Lung: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(PLK3 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-PRK antibody

Cytokine-inducible kinase is a Polo serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis.

Product Categories/Family for anti-PRK antibody

Family: Protein Kinase; Subfamily: Polo

NCBI and Uniprot Product Information

• NCBI GI #: 41872374
• NCBI GeneID: 1263
• NCBI Accession #: NP_004064.2
• NCBI GenBank Nucleotide #: NM_004073.2
• UniProt Accession #: Q9H4B4; Q15767; Q5JR99; Q96CV1
• Molecular Weight: 71,629 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK3
• NCBI Official Synonym Full Names: polo-like kinase 3
• NCBI Official Symbol: PLK3
• NCBI Official Synonym Symbols: CNK; FNK; PRK
• NCBI Protein Information: serine/threonine-protein kinase PLK3; PLK-3; FGF-inducible kinase; cytokine-inducible kinase; proliferation-related kinase; cytokine-inducible serine/threonine-protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase PLK3
• UniProt Gene Name: PLK3
• UniProt Synonym Gene Names: CNK; FNK; PRK; PLK-3
• UniProt Entry Name: PLK3_HUMAN

NCBI Description

Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.

Protein type: Protein kinase, Other; Kinase, protein; Nucleolus; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: centrosome; Golgi stack; cell soma; cytoplasm; dendrite; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; ATP binding

Biological Process: apoptosis; mitotic cell cycle checkpoint; negative regulation of transcription from RNA polymerase II promoter; response to osmotic stress; endomitotic cell cycle; protein amino acid phosphorylation; regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; response to radiation; response to reactive oxygen species; protein kinase B signaling cascade; G2/M transition of mitotic cell cycle; regulation of cell division; response to DNA damage stimulus; G1/S transition of mitotic cell cycle; negative regulation of apoptosis