Rabbit anti-Human PRICKLE1 Polyclonal Antibody | anti-PRICKLE1 antibody

PRICKLE1, CT (PRICKLE1, RILP, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1) (PE)

Cat. Num. AAA6337214

• Gene Names: PRICKLE1; RILP; EPM1B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRICKLE1; Polyclonal Antibody; CT (PRICKLE1; RILP; Prickle-like protein 1; REST/NRSF-interacting LIM domain protein 1) (PE); REST/NRSF-interacting LIM domain protein 1; anti-PRICKLE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PRICKLE1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRICKLE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 677-704 amino acids selected from the C-terminal region of human PRICKLE1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PRICKLE1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the PRICKLE1 antibody detected the PRICKLE1 protein (arrow).)

Related Product Information for anti-PRICKLE1 antibody

This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3.

Product Categories/Family for anti-PRICKLE1 antibody

Antibodies; Wnt Proteins

References

Smith, N.L., et al. Circ Cardiovasc Genet 3(3):256-266(2010). Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Perry, J.R., et al. Diabetes 58(6):1463-1467(2009). Narimatsu, M., et al. Cell 137(2):295-307(2009). Shimojo, M. J. Biol. Chem. 283(50):34880-34886(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 222136680
• NCBI GeneID: 144165
• NCBI Accession #: NP_001138353.1
• NCBI GenBank Nucleotide #: NM_001144881.1
• UniProt Accession #: Q96MT3; Q14C83; Q71QF8; Q96N00
• Molecular Weight: 94,300 Da
• NCBI Official Full Name: prickle-like protein 1
• NCBI Official Synonym Full Names: prickle planar cell polarity protein 1
• NCBI Official Symbol: PRICKLE1
• NCBI Official Synonym Symbols: RILP; EPM1B
• NCBI Protein Information: prickle-like protein 1
• UniProt Protein Name: Prickle-like protein 1
• Protein Family: Prickle-like protein
• UniProt Gene Name: PRICKLE1
• UniProt Synonym Gene Names: RILP
• UniProt Entry Name: PRIC1_HUMAN

NCBI Description

This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]

Uniprot Description

PRICKLE1: Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure. Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor. Defects in PRICKLE1 are the cause of progressive myoclonic epilepsy type 1B (EPM1B). EPM1B is an autosomal recessive disorder characterized by myoclonus that progresses in severity over time, tonic-clonic seizures and ataxia. Defects in PRICKLE1 may be a cause of susceptibility to neural tube defects (NTD). Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components. Belongs to the prickle / espinas / testin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: nuclear membrane; cytosol; nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: positive regulation of protein ubiquitination; protein import into nucleus; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; neural tube closure; negative regulation of transcription, DNA-dependent

Disease: Epilepsy, Progressive Myoclonic 1b