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Western Blot (WB) (Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 151 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit PRG4 Polyclonal Antibody | anti-PRG4 antibody

PRG4 Antibody (N-Term)

Gene Names
PRG4; MSF; SZP; CACP; HAPO; JCAP
Reactivity
Human
Predicted: Human
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
PRG4; Polyclonal Antibody; PRG4 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Proteoglycan 4; Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycan; Proteoglycan 4 C-terminal part; MSF; SZP; anti-PRG4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted: Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
1363
Applicable Applications for anti-PRG4 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IF: 1:25
WB: 1:500
Antigen Type
Synthesized Peptide
Antigen Source
Human
Tissue Location
Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed.
Cellular Location
Secreted.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 151 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 151 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PRG4 antibody
Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
Product Categories/Family for anti-PRG4 antibody
References
Turner K.J.,et al. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Jones A.R.,et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Flannery C.R.,et al.Biochem. Biophys. Res. Commun. 254:535-541(1999).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
151077
NCBI Official Full Name
proteoglycan 4 isoform B preproprotein
NCBI Official Synonym Full Names
proteoglycan 4
NCBI Official Symbol
PRG4
NCBI Official Synonym Symbols
MSF; SZP; CACP; HAPO; JCAP
NCBI Protein Information
proteoglycan 4
UniProt Protein Name
Proteoglycan 4
Protein Family
UniProt Gene Name
PRG4
UniProt Synonym Gene Names
MSF; SZP

NCBI Description

The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.MiscellaneousDifferent forms varying in molecular weight have been observed. Such forms are possibly due to different levels of glycosylation and protein cleavage ().

Research Articles on PRG4

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Product Notes

The PRG4 prg4 (Catalog #AAA9231305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRG4 Antibody (N-Term) reacts with Human Predicted: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). IF: 1:25 WB: 1:500. Researchers should empirically determine the suitability of the PRG4 prg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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