Western Blot (WB)
(Western blot analysis of PREX1 using HuvEc whole cell lysates)
Rabbit PREX1 Polyclonal Antibody | anti-PREX1 antibody
PREX1 Antibody
Gene Names
PREX1; P-REX1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PREX1; Polyclonal Antibody; PREX1 Antibody; 4; 5)-dependent Rac exchanger 1; KIAA1415; OTTHUMP00000031228; P REX1; P Rex1 protein like; P-Rex1; Phosphatidylinositol 3; 5 trisphosphate dependent Rac exchange factor 1; 5 trisphosphate dependent Rac exchanger 1; 5 trisphosphate dependent Rac exchanger 1 protein; 5-trisphosphate-dependent Rac exchanger 1 protein; Prex1; PREX1_HUMAN; PtdIns(3; 5) dependent Rac exchanger 1; SET domain containing 6; Setd6; anti-PREX1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PREX1 antibody detects endogenous levels of total PREX1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1659
Applicable Applications for anti-PREX1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human PREX1
Subcellular Location
Cytoplasm > Cytosol. Cell Membrane. Mainly Cytosolic. Some amount is apparently associated to the plasma membrane.
Tissue Specificity
Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Zebrafish (80%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PREX1 using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of PREX1 using HuvEc whole cell lysates)
Immunohistochemistry (IHC)
(MBS9610057 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9610057 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PREX1 antibody
Function: Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3, 4, 5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.
Subunit Structure: Interacts preferentially with RAC2. Interacts with RAC1. Interacts with AUTS2.
Subunit Structure: Interacts preferentially with RAC2. Interacts with RAC1. Interacts with AUTS2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 186 kDa
Predicted: 187 kDa
Predicted: 187 kDa
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
NCBI Official Synonym Full Names
phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1
NCBI Official Symbol
PREX1
NCBI Official Synonym Symbols
P-REX1
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein
UniProt Gene Name
PREX1
UniProt Synonym Gene Names
KIAA1415; P-Rex1; PtdIns(3,4,5)-dependent Rac exchanger 1
NCBI Description
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.
Research Articles on PREX1
Similar Products
Product Notes
The PREX1 prex1 (Catalog #AAA9610057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PREX1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PREX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the PREX1 prex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PREX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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