Western Blot (WB)
(PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).)
Rabbit anti-Human PREX1 Polyclonal Antibody | anti-PREX1 antibody
PREX1 Antibody (C-term)
Western Blot (WB)
(PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).)
Western Blot (WB)
(PREX1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PREX1 antibody detected the PREX1 protein (arrow).)
Immunohistochemistry (IHC)
(PREX1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PREX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PREX1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PREX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PREX1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PREX1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
nucleotide exchange factor for the RHO family of small GTP-binding
proteins (RACs). It has been shown to bind to and activate RAC1 by
exchanging bound GDP for free GTP. The encoded protein, which is
found mainly in the cytoplasm, is activated by
phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma
subunits of heterotrimeric G proteins.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Nie, B., et al. Cell. Signal. 22(5):770-782(2010)
Carretero-Ortega, J., et al. Mol. Pharmacol. 77(3):435-442(2010)
Ledezma-Sanchez, B.A., et al. Biochem. Biophys. Res. Commun. 391(4):1647-1652(2010)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008]
Uniprot Description
PREX1: Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils. Interacts preferentially with RAC2. Interacts with RAC1. Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 20q13.13
Cellular Component: growth cone; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; cytosol
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; enzyme binding; phospholipid binding
Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; regulation of actin filament polymerization; neutrophil activation; actin filament polymerization; regulation of dendrite development; superoxide metabolic process; T cell differentiation
Research Articles on PREX1
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Product Notes
The PREX1 prex1 (Catalog #AAA9208975) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1134-1163. The PREX1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PREX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the PREX1 prex1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PREX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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