Western Blot (WB)
((0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PRDX1 Polyclonal Antibody | anti-PRDX1 antibody
Goat anti-PRDX1 Antibody
Western Blot: Approx.23 to 24kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 22.1kDa according to NP_859048.1). Recommended concentration: 0.01-0.03ug/ml.
IHC: Paraffin embedded Human Thyroid Gland and Placenta. Recommended concentration: 2.5ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
IHC
((2.5?g/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC
((2.5?g/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC
((2.5?g/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC
((2.5?g/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]
Uniprot Description
PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.
Protein type: EC 1.11.1.15; Oxidoreductase; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: cytoplasm; cytosol; extracellular space; melanosome; mitochondrion; myelin sheath; nucleus
Molecular Function: identical protein binding; peroxidase activity; protein binding; thioredoxin peroxidase activity
Biological Process: cell proliferation; erythrocyte homeostasis; hydrogen peroxide catabolic process; natural killer cell mediated cytotoxicity; regulation of NF-kappaB import into nucleus; regulation of stress-activated MAPK cascade; removal of superoxide radicals; response to reactive oxygen species; retinal homeostasis; skeletal development
Research Articles on PRDX1
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Product Notes
The PRDX1 prdx1 (Catalog #AAA422240) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PRDX1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PRDX1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx.23 to 24kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 22.1kDa according to NP_859048.1). Recommended concentration: 0.01-0.03ug/ml. IHC: Paraffin embedded Human Thyroid Gland and Placenta. Recommended concentration: 2.5ug/ml. Researchers should empirically determine the suitability of the PRDX1 prdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SDPKRTIAQD YG. It is sometimes possible for the material contained within the vial of "PRDX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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