Rabbit anti-Human PRDM6 Polyclonal Antibody | anti-PRDM6 antibody

PRDM6, NT (PRDM6, PFM3, Putative histone-lysine N-methyltransferase PRDM6, PR domain zinc finger protein 6, PR domain-containing protein 6) (MaxLight 750)

Cat. Num. AAA6337092

• Gene Names: PRDM6; PDA3; KMT8C; PRISM
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRDM6; Polyclonal Antibody; NT (PRDM6; PFM3; Putative histone-lysine N-methyltransferase PRDM6; PR domain zinc finger protein 6; PR domain-containing protein 6) (MaxLight 750); PR domain-containing protein 6; anti-PRDM6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-PRDM6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRDM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human PRDM6.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-PRDM6 antibody

Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression (By similarity).

Product Categories/Family for anti-PRDM6 antibody

Antibodies; Enzymes; Methyltransferase

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Vasan, R.S., et al. JAMA 302(2):168-178(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 210031233
• NCBI GeneID: 93166
• NCBI Accession #: NP_001129711.1
• NCBI GenBank Nucleotide #: NM_001136239.3
• UniProt Accession #: Q9NQX0; Q9NQW9; B5MCJ4
• Molecular Weight: 46,635 Da
• NCBI Official Full Name: putative histone-lysine N-methyltransferase PRDM6
• NCBI Official Synonym Full Names: PR/SET domain 6
• NCBI Official Symbol: PRDM6
• NCBI Official Synonym Symbols: PDA3; KMT8C; PRISM
• NCBI Protein Information: putative histone-lysine N-methyltransferase PRDM6
• UniProt Protein Name: Putative histone-lysine N-methyltransferase PRDM6
• Protein Family: Putative histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM6
• UniProt Synonym Gene Names: PFM3

NCBI Description

The protein encoded by this gene is a transcriptional repressor and a member of the PRDM family. Family members contain a PR domain and multiple zinc-finger domains. The encoded protein is involved in regulation of vascular smooth muscle cells (VSMC) contractile proteins. Mutations in this gene result in patent ductus arteriosus 3 (PDA3). [provided by RefSeq, Apr 2017]

Uniprot Description

PRDM6: Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression. Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells. Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a. According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell development/differentiation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleus

Molecular Function: histone-lysine N-methyltransferase activity; metal ion binding; nucleic acid binding; protein homodimerization activity

Biological Process: negative regulation of smooth muscle cell differentiation; negative regulation of transcription, DNA-dependent; neurogenesis; transcription, DNA-dependent

Disease: Patent Ductus Arteriosus 3