Rabbit PRAS40 Polyclonal Antibody | anti-PRAS40 antibody

PRAS40 (Phospho-Ser212) Antibody

Cat. Num. AAA9429423

• Gene Names: AKT1S1; Lobe; PRAS40
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: PRAS40; Polyclonal Antibody; PRAS40 (Phospho-Ser212) Antibody; 40 kDa proline rich AKT substrate; 40 kDa proline-rich AKT substrate; AKT1 S1; AKT1 substrate 1 (proline rich); AKT1 substrate 1; AKT1S 1; AKT1S1; AKTS1_HUMAN; Lobe; MGC2865; PRAS 40; PRAS; Proline rich akt substrate; Proline rich Akt substrate 40 kDa; Proline-rich AKT1 substrate 1;; anti-PRAS40 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-PRAS40 (Phospho-Ser212) Antibody detects endogenous levels of PRAS40 only when phosphorylated at Ser212
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/mL (varies by lot)
• Sequence Length: 256

• Applicable Applications for anti-PRAS40 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human PRAS40 around the phosphorylation site of Ser212
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Phospho-PRAS40 (Ser212) in lysates of HepG2 PDGF 50ng/ml 30', using Phospho-PRAS40 (Ser212) Antibody(13271).)

Product Categories/Family for anti-PRAS40 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 148806906
• NCBI GeneID: 84335
• NCBI Accession #: NP_001092102.1
• NCBI GenBank Nucleotide #: NM_001098632.2
• UniProt Accession #: Q96B36; Q96BI4; Q96IK7; Q96NG2; Q9BWR5; A8MTQ1; B2RE93; J3KPM3
• Molecular Weight: 27
• NCBI Official Full Name: proline-rich AKT1 substrate 1 isoform b
• NCBI Official Synonym Full Names: AKT1 substrate 1
• NCBI Official Symbol: AKT1S1
• NCBI Official Synonym Symbols: Lobe; PRAS40
• NCBI Protein Information: proline-rich AKT1 substrate 1
• UniProt Protein Name: Proline-rich AKT1 substrate 1
• UniProt Gene Name: AKT1S1

NCBI Description

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

Uniprot Description

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.