Western Blot (WB)
(Western blot analysis of PRAMEF3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. PRAMEF3 Antibody (C-term) (arrow) was detected using the purified Pab.)
Rabbit anti-Human PRAMEF3 Polyclonal Antibody | anti-PRAMEF3 antibody
PRAMEF3, CT (PRAMEF3, PRAME family member 3) (PE)
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PRAMEF3; Polyclonal Antibody; CT (PRAMEF3; PRAME family member 3) (PE); PRAME family member 3; anti-PRAMEF3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-PRAMEF3 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PRAMEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 427-457 amino acids from the C-terminal region of human PRAMEF3.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PRAMEF3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. PRAMEF3 Antibody (C-term) (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PRAMEF3 Antibody (C-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H460 cell line lysate. PRAMEF3 Antibody (C-term) (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(PRAMEF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PRAMEF3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRAMEF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-PRAMEF3 antibody
References
General References: 1. Ikeda H, Lethe B, Lehmann F, van Baren N, Baurain JF, de Smet C, Chambost H, Vitale M, Moretta A, Boon T, Coulie PG (Mar 1997). "Characterization of an antigen that is recognized on a melanoma showing partial HLA loss by CTL expressing an NK inhibitory receptor". Immunity 6 (2): 199-208. doi:10.1016/S1074-7613(00)80426-4. PMID 9047241. 2. Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP, et al. (Dec 1999). "The DNA sequence of human chromosome 22". Nature 402 (6761): 489-95. doi:10.1038/990031. PMID 10591208. 3. "Entrez Gene: PRAME preferentially expressed antigen in melanoma".
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PRAMEF3 (Catalog #AAA6336983) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRAMEF3, CT (PRAMEF3, PRAME family member 3) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRAMEF3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRAMEF3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAMEF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
