Western Blot (WB)
(Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab.)
Rabbit anti-Human PRAME Polyclonal Antibody | anti-PRAME antibody
PRAME, CT (Preferentially Expressed Antigen of Melanoma, Melanoma Antigen Preferentially Expressed in Tumors, MAPE, CT130, OPA Interacting Protein 4, OPA-interacting Protein 4, Opa-interacting Protein OIP4, OIP4) (MaxLight 550)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MAPE Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
Uniprot Description
PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family.
Protein type: Nuclear receptor co-regulator; Cancer Testis Antigen (CTA); Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 22q11.22
Cellular Component: plasma membrane; nucleus
Molecular Function: protein binding; retinoic acid receptor binding
Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; transcription, DNA-dependent; apoptosis; regulation of growth; positive regulation of cell proliferation; cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
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Product Notes
The PRAME prame (Catalog #AAA6494161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRAME, CT (Preferentially Expressed Antigen of Melanoma, Melanoma Antigen Preferentially Expressed in Tumors, MAPE, CT130, OPA Interacting Protein 4, OPA-interacting Protein 4, Opa-interacting Protein OIP4, OIP4) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRAME can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRAME prame for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRAME, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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