Western Blot (WB)
(Western blot analysis of PPP3R2 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PPP3R2 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse PPP3R2 Polyclonal Antibody | anti-PPP3R2 antibody
PPP3R2, NT (PPP3R2, CBLP, PPP3RL, Calcineurin subunit B type 2, Calcineurin B-like protein, Calcineurin BII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform) (APC)
Gene Names
PPP3R2; PPP3RL
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PPP3R2; Polyclonal Antibody; NT (PPP3R2; CBLP; PPP3RL; Calcineurin subunit B type 2; Calcineurin B-like protein; Calcineurin BII; PPP3R1-like; Protein phosphatase 2B regulatory subunit 2; Protein phosphatase 3 regulatory subunit B beta isoform) (APC); Protein phosphatase 3 regulatory subunit B beta isoform; anti-PPP3R2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PPP3R2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PPP3R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~38 amino acids from the N-terminal region of human PPP3R2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PPP3R2 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PPP3R2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PPP3R2 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PPP3R2 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PPP3R2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PPP3R2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PPP3R2 Antibody (N-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PPP3R2 Antibody (N-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PPP3R2 antibody
PPP3R2 is regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. This protein confers calcium sensitivity (By similarity).
Product Categories/Family for anti-PPP3R2 antibody
References
He, Z.H., et al. Eur. J. Appl. Physiol. (2010). In press: Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009). Chow, R., et al. Biochem. Biophys. Res. Commun. 363(3):506-512(2007). Saeki, M., et al. J. Biol. Chem. 282(16):11786-11794(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
170
NCBI Official Full Name
Calcineurin subunit B type 2
NCBI Official Synonym Full Names
protein phosphatase 3, regulatory subunit B, beta
NCBI Official Symbol
PPP3R2
NCBI Official Synonym Symbols
PPP3RL
NCBI Protein Information
calcineurin subunit B type 2; CBLP; CNBII; calcineurin BII; calcineurin B-like protein; calcineurin B, type II (19kDa); protein phosphatase 2B regulatory subunit 2; protein phosphatase 3 regulatory subunit B beta isoform; protein phosphatase 3 (formerly 2
UniProt Protein Name
Calcineurin subunit B type 2
Protein Family
UniProt Gene Name
PPP3R2
UniProt Synonym Gene Names
CBLP; PPP3RL; CBLP; CNBII
UniProt Entry Name
CANB2_HUMAN
Uniprot Description
PPP3R2: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family.
Protein type: Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 9q31.1
Molecular Function: calcium ion binding
Research Articles on PPP3R2
Similar Products
Product Notes
The PPP3R2 ppp3r2 (Catalog #AAA6336710) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP3R2, NT (PPP3R2, CBLP, PPP3RL, Calcineurin subunit B type 2, Calcineurin B-like protein, Calcineurin BII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP3R2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPP3R2 ppp3r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP3R2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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