Rabbit anti-Human PPP3CA Polyclonal Antibody | anti-PPP3CA antibody

PPP3CA, CT (PPP3CA, CALNA, CNA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform) (PE)

Cat. Num. AAA6336697

• Gene Names: PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP3CA; Polyclonal Antibody; CT (PPP3CA; CALNA; CNA; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform) (PE); Calmodulin-dependent calcineurin A subunit alpha isoform; anti-PPP3CA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PPP3CA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP3CA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 437-466 amino acids from the C-terminal region of human PPP3CA.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPP3CA Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the PPP3CA antibody detected the PPP3CA protein (arrow).)

Related Product Information for anti-PPP3CA antibody

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

Product Categories/Family for anti-PPP3CA antibody

Antibodies; Calmodulin

References

He, Z.H., et al. Eur. J. Appl. Physiol. 110(4):761-767(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Chiocco, M.J., et al. Subst Use Misuse 45(11):1809-1826(2010). Yang, D., et al. Int. J. Mol. Med. 26(1):159-164(2010). Bollo, M., et al. PLoS ONE 5 (8), E11925 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 194688146
• NCBI GeneID: 5530
• NCBI Accession #: NP_001124163.1; NP_000935.1
• NCBI GenBank Nucleotide #: NM_001130691
• UniProt Accession #: Q08209; Q8TAW9; A8K3B7; A8W6Z7; A8W6Z8; B5BUA2
• Molecular Weight: ~83 kDa
• NCBI Official Full Name: Homo sapiens protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP3CA
• NCBI Official Synonym Symbols: CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatas
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP3CA
• UniProt Synonym Gene Names: CALNA; CNA
• UniProt Entry Name: PP2BA_HUMAN

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; Z disc; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein dimerization activity; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; protein serine/threonine phosphatase activity; drug binding; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; calcineurin-NFAT signaling pathway; transition between fast and slow fiber; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle