Rabbit anti-Human PPP2R5D Polyclonal Antibody | anti-PPP2R5D antibody

PPP2R5D, NT (PPP2R5D, Serine/threonine-protein phosphatase 2A 56kD regulatory subunit delta isoform, PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform R5-delta) (FITC)

Cat. Num. AAA6336679

• Gene Names: PPP2R5D; B56D; MRD35; B56delta
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP2R5D; Polyclonal Antibody; NT (PPP2R5D; Serine/threonine-protein phosphatase 2A 56kD regulatory subunit delta isoform; PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta) (FITC); PP2A B subunit isoform R5-delta; anti-PPP2R5D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 496

• Applicable Applications for anti-PPP2R5D antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP2R5D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-91 amino acids from the N-terminal region of human PPP2R5D.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP2R5D Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PPP2R5D antibody detected the PPP2R5D protein (arrow).)

Related Product Information for anti-PPP2R5D antibody

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-PPP2R5D antibody

Antibodies; Enzymes; Phosphatase

References

Yu, U.Y., et al. BMB Rep 43(4):263-267(2010). Reece, K.M., et al. Biochem. Biophys. Res. Commun. 386(4):582-587(2009). Forester, C.M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(50):19867-19872(2007). Sablina, A.A., et al. Cell 129(5):969-982(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 31083288
• NCBI GeneID: 5528
• NCBI Accession #: NP_851308.1
• NCBI GenBank Nucleotide #: NP_851308.1
• UniProt Accession #: Q14738
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform isoform 3
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit B'delta
• NCBI Official Symbol: PPP2R5D
• NCBI Official Synonym Symbols: B56D; MRD35; B56delta
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2R5D
• UniProt Entry Name: 2A5D_HUMAN

NCBI Description

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2R5D: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. By retinoic acid; in neuroblastoma cell lines. Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. Belongs to the phosphatase 2A regulatory subunit B56 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytosol; nucleus

Molecular Function: protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity

Biological Process: nervous system development; glycolysis; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; glucose metabolic process; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; dephosphorylation; regulation of catalytic activity; carbohydrate metabolic process; energy reserve metabolic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Mental Retardation, Autosomal Dominant 35