Rabbit PPP2R1A Polyclonal Antibody | anti-PPP2R1A antibody

PPP2R1A (Serine/threonine-protein phosphatase 2A 65kD regulatory subunit A alpha isoform, PR65A, PP2AAALPHA, PP2A-Aalpha) (AP)

Cat. Num. AAA6432645

• Gene Names: PPP2R1A; MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PPP2R1A; Polyclonal Antibody; PPP2R1A (Serine/threonine-protein phosphatase 2A 65kD regulatory subunit A alpha isoform; PR65A; PP2AAALPHA; PP2A-Aalpha) (AP); anti-PPP2R1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PPP2R1A. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PPP2R1A antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PPP2R1A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PPP2R1A antibody.)

Product Categories/Family for anti-PPP2R1A antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 21361399
• NCBI GeneID: 5518
• NCBI Accession #: NP_055040.2
• NCBI GenBank Nucleotide #: NM_014225.5
• UniProt Accession #: P30153; Q13773; Q6ICQ3; Q96DH3
• Molecular Weight: 65,309 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit A, alpha
• NCBI Official Symbol: PPP2R1A
• NCBI Official Synonym Symbols: MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Gene Name: PPP2R1A
• UniProt Entry Name: 2AAA_HUMAN

Uniprot Description

PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: chromosome, pericentric region; cytosol; protein phosphatase type 2A complex

Molecular Function: antigen binding; protein binding; protein heterodimerization activity; protein phosphatase regulator activity

Biological Process: apoptosis; chromosome segregation; G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation; protein complex assembly

Disease: Mental Retardation, Autosomal Dominant 36