Rabbit anti-Human PPP1R9B Polyclonal Antibody | anti-PPP1R9B antibody

PPP1R9B, NT (PPP1R9B, PPP1R6, Neurabin-2, Protein phosphatase 1 regulatory subunit 9B, Spinophilin, Neurabin-II) (Biotin)

Cat. Num. AAA6336612

• Gene Names: PPP1R9B; Spn; SPINO; PPP1R6; PPP1R9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP1R9B; Polyclonal Antibody; NT (PPP1R9B; PPP1R6; Neurabin-2; Protein phosphatase 1 regulatory subunit 9B; Spinophilin; Neurabin-II) (Biotin); Neurabin-II; anti-PPP1R9B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PPP1R9B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP1R9B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the N-terminal region of human PPP1R9B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP1R9B Antibody (N-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PPP1R9B antibody detected the PPP1R9B protein (arrow).)

Related Product Information for anti-PPP1R9B antibody

Spinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system (Allen et al., 1997 [PubMed 9275233]).

Product Categories/Family for anti-PPP1R9B antibody

Antibodies; Enzymes; Phosphatase

References

Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010): Rajagopal, S., et al. J. Biol. Chem. 285(23):18060-18071(2010). Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009). Sagara, M., et al. Oncogene 28(10):1357-1365(2009). Meng, X., et al. Eur. J. Immunol. 39(2):552-560(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 140972063
• NCBI GeneID: 84687
• NCBI Accession #: NP_115984.3
• NCBI GenBank Nucleotide #: NM_032595.4
• UniProt Accession #: Q96SB3; Q8TCR9
• Molecular Weight: 89,192 Da
• NCBI Official Full Name: neurabin-2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 9B
• NCBI Official Symbol: PPP1R9B
• NCBI Official Synonym Symbols: Spn; SPINO; PPP1R6; PPP1R9
• NCBI Protein Information: neurabin-2; neurabin-II; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin
• UniProt Protein Name: Neurabin-2
• Protein Family: Neurabin
• UniProt Gene Name: PPP1R9B
• UniProt Synonym Gene Names: PPP1R6
• UniProt Entry Name: NEB2_HUMAN

NCBI Description

This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014]

Uniprot Description

Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain.

Protein type: Protein phosphatase, regulatory subunit; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: nucleoplasm; cortical actin cytoskeleton; adherens junction; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; protein phosphatase type 1 complex; filopodium

Molecular Function: protein binding; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding

Biological Process: regulation of synaptic transmission; regulation of protein amino acid phosphorylation; cell migration; regulation of cell growth by extracellular stimulus; filopodium formation; RNA splicing; regulation of exit from mitosis; calcium-mediated signaling; actin filament organization; regulation of cell proliferation; negative regulation of catalytic activity; dendrite development; negative regulation of cell growth; cell cycle arrest