Rabbit PPP1R8 Polyclonal Antibody | anti-PPP1R8 antibody

PPP1R8 (Protein Phosphatase 1 Regulatory Inhibitor Subunit 8, ARD1, ARD-1, Nuclear Inhibitor of Protein Phosphatase 1, NIPP1, NIPP-1, PRO2047) (Biotin)

Cat. Num. AAA6432614

• Gene Names: PPP1R8; ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• Reactivity: Human, Mouse, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PPP1R8; Polyclonal Antibody; PPP1R8 (Protein Phosphatase 1 Regulatory Inhibitor Subunit 8; ARD1; ARD-1; Nuclear Inhibitor of Protein Phosphatase 1; NIPP1; NIPP-1; PRO2047) (Biotin); anti-PPP1R8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PPP1R8. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PPP1R8 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1:20-1:50; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human PPP1R8.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PPP1R8 antibody.)

Product Categories/Family for anti-PPP1R8 antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 4506015
• NCBI GeneID: 5511
• NCBI Accession #: NP_002704.1
• NCBI GenBank Nucleotide #: NM_002713.3
• UniProt Accession #: Q12972; Q5TEJ2; Q5TEJ4; Q5TIF2; Q6PKF6; Q9UBH1; Q9UBZ0
• Molecular Weight: 38,479 Da
• NCBI Official Full Name: nuclear inhibitor of protein phosphatase 1 isoform gamma
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 8
• NCBI Official Symbol: PPP1R8
• NCBI Official Synonym Symbols: ARD1; ARD-1; NIPP1; NIPP-1; PRO2047
• NCBI Protein Information: nuclear inhibitor of protein phosphatase 1; RNase E; activator of RNA decay; nuclear subunit of PP-1; protein phosphatase 1 regulatory subunit 8; nuclear inhibitor of protein phosphatase-1 alpha; protein phosphatase 1, regulatory (inhibitor) subunit 8
• UniProt Protein Name: Nuclear inhibitor of protein phosphatase 1
• Protein Family: Nuclear inhibitor of protein phosphatase
• UniProt Gene Name: PPP1R8
• UniProt Synonym Gene Names: ARD1; NIPP1; NIPP-1
• UniProt Entry Name: PP1R8_HUMAN

NCBI Description

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

Uniprot Description

NIPP-1: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; EC 3.1.4.-; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit; Ribonuclease

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; ribonuclease E activity; RNA binding; protein phosphatase type 1 regulator activity; endonuclease activity; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; RNA catabolic process; negative regulation of catalytic activity; mRNA processing