Rabbit anti-Human PPP1R3A Polyclonal Antibody | anti-PPP1R3A antibody

PPP1R3A, ID (PPP1R3A, PP1G, Protein phosphatase 1 regulatory subunit 3A, Protein phosphatase 1 glycogen-associated regulatory subunit, Protein phosphatase type-1 glycogen targeting subunit) (Azide free) (HRP)

Cat. Num. AAA6336548

• Gene Names: PPP1R3A; GM; PP1G; PPP1R3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP1R3A; Polyclonal Antibody; ID (PPP1R3A; PP1G; Protein phosphatase 1 regulatory subunit 3A; Protein phosphatase 1 glycogen-associated regulatory subunit; Protein phosphatase type-1 glycogen targeting subunit) (Azide free) (HRP); Protein phosphatase type-1 glycogen targeting subunit; anti-PPP1R3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPP1R3A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP1R3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-786 amino acids from the Central region of human PPP1R3A.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP1R3A Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PPP1R3A antibody detected the PPP1R3A protein (arrow).)

Flow Cytometry (FC/FACS)

(PPP1R3A Antibody (Center) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PPP1R3A antibody

The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq].

Product Categories/Family for anti-PPP1R3A antibody

Antibodies; Enzymes; Phosphatase

References

Yoshida, T., et al. Int. J. Mol. Med. 24(2):233-246(2009). Yoshida, T., et al. Genomics 93(3):221-226(2009). Gieger, C., et al. PLoS Genet. 4 (11), E1000282 (2008): Savage, D.B., et al. PLoS Med. 5 (1), E27 (2008): Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 167614502
• NCBI GeneID: 5506
• NCBI Accession #: NP_002702.2
• NCBI GenBank Nucleotide #: NM_002711.3
• UniProt Accession #: Q16821; O43476; Q75LN8; Q7KYM8; Q86UI6; A0AVQ2; A4D0T6
• Molecular Weight: 125,767 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 3A
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 3A
• NCBI Official Symbol: PPP1R3A
• NCBI Official Synonym Symbols: GM; PP1G; PPP1R3
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 3A; RG1; protein phosphatase 1 regulatory subunit GM; serine /threonine specific protein phosphatase; protein phosphatase type-1 glycogen targeting subunit; protein phosphatase 1, regulatory (inhibitor) subunit 3A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 3A
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R3A
• UniProt Synonym Gene Names: PP1G; RG1
• UniProt Entry Name: PPR3A_HUMAN

NCBI Description

The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP1R3A: Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase. Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen. Skeletal muscle and heart. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q31.1

Cellular Component: integral to membrane

Biological Process: glycogen metabolic process

Disease: Lipodystrophy, Familial Partial, Type 3