Rabbit anti-Human PPP1R2 Polyclonal Antibody | anti-PPP1R2 antibody

PPP1R2, ID (PPP1R2, IPP2, Protein phosphatase inhibitor 2) (Azide free) (HRP)

Cat. Num. AAA6336526

• Gene Names: PPP1R2; IPP2; IPP-2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP1R2; Polyclonal Antibody; ID (PPP1R2; IPP2; Protein phosphatase inhibitor 2) (Azide free) (HRP); Protein phosphatase inhibitor 2; anti-PPP1R2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PPP1R2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP1R2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-148 amino acids from the Central region of human PPP1R2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PPP1R2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PPP1R2 antibody detected the PPP1R2 protein (arrow).)

Product Categories/Family for anti-PPP1R2 antibody

Antibodies; Enzymes; Phosphatase

References

Wang, W., et al. Mol. Biol. Cell 19(11):4852-4862(2008). Wang, W., et al. BMC Cell Biol. 9, 62 (2008): Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 5453946
• NCBI GeneID: 5504
• NCBI Accession #: NP_006232.1
• NCBI GenBank Nucleotide #: NM_006241.4
• UniProt Accession #: P41236
• Molecular Weight: 23,015 Da
• NCBI Official Full Name: protein phosphatase inhibitor 2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 2
• NCBI Official Symbol: PPP1R2
• NCBI Official Synonym Symbols: IPP2; IPP-2
• NCBI Protein Information: protein phosphatase inhibitor 2; phosphoprotein phosphatase
• UniProt Protein Name: Protein phosphatase inhibitor 2
• Protein Family: Protein phosphatase inhibitor
• UniProt Gene Name: PPP1R2
• UniProt Synonym Gene Names: IPP2; IPP-2
• UniProt Entry Name: IPP2_HUMAN

Uniprot Description

PPP1R2: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Inhibitor; Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q29

Molecular Function: protein binding; type 1 serine/threonine specific protein phosphatase inhibitor activity

Biological Process: glycogen metabolic process; generation of precursor metabolites and energy; negative regulation of catalytic activity; regulation of signal transduction; regulation of phosphoprotein phosphatase activity