Western Blot (WB)
(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS648378. This demonstrates that MBS648378 detected the PPM1D protein (arrow).)
Rabbit anti-Human PPM1D Polyclonal Antibody | anti-PPM1D antibody
PPM1D, ID (PPM1D, WIP1, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1) (MaxLight 550)
Gene Names
PPM1D; WIP1; PP2C-DELTA
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
PPM1D; Polyclonal Antibody; ID (PPM1D; WIP1; Protein phosphatase 1D; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1) (MaxLight 550); p53-induced protein phosphatase 1; anti-PPM1D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PPM1D.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Applicable Applications for anti-PPM1D antibody
Western Blot (WB), FLISA
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 183-212 in the central region of human PPM1D, UniProt Accession #O15297. Species sequence homology: mouse
Conjugate
MaxLight550
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS648378. This demonstrates that MBS648378 detected the PPM1D protein (arrow).)
Western Blot (WB)
(Western Blot analysis in Hela cell line lysates (35ug/lane) usingMBS648378. This demonstrates that MBS648378 detected the PPM1D protein (arrow).)
Testing Data
(All lanes: MBS648378 (1:1000) Lane 1: ZR-75-1 whole cell lysate Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 67kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data
(All lanes: MBS648378 (1:1000) Lane 1: ZR-75-1 whole cell lysate Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 67kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-PPM1D antibody
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
Product Categories/Family for anti-PPM1D antibody
References
1. Zhang, X., et al. Cancer Res. 70(18):7176-7186(2010). 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Moon, S.H., et al. J. Biol. Chem. 285(17):12935-12947(2010). 4. Macurek, L., et al. Oncogene 29(15):2281-2291(2010). 5. Yang, D.H., et al. Zhonghua Yi Xue Za Zhi 90(8):519-522(2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,211 Da
NCBI Official Full Name
protein phosphatase 1D
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent, 1D
NCBI Official Symbol
PPM1D
NCBI Official Synonym Symbols
WIP1; PP2C-DELTA
NCBI Protein Information
protein phosphatase 1D
UniProt Protein Name
Protein phosphatase 1D
Protein Family
UniProt Gene Name
PPM1D
UniProt Synonym Gene Names
WIP1; PP2C-delta
UniProt Entry Name
PPM1D_HUMAN
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Product Notes
The PPM1D ppm1d (Catalog #AAA6336386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPM1D, ID (PPM1D, WIP1, Protein phosphatase 1D, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1) (MaxLight 550) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPM1D ppm1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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