Rabbit PPM1D Polyclonal Antibody | anti-PPM1D antibody

PPM1D Antibody

Cat. Num. AAA153719

• Gene Names: PPM1D; WIP1; PP2C-DELTA
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PPM1D antibody is affinity chromatography purified via peptide column.
• Synonyms: PPM1D; Polyclonal Antibody; PPM1D Antibody; Protein phosphatase Mg2+/Mn2+ dependent 1D; p53-induced protein phosphatase 1; PP2C-DELTA; Protein phosphatase 1D; WIP1 ; anti-PPM1D antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.
• Purity/Purification: PPM1D antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 605

• Applicable Applications for anti-PPM1D antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D.
• Buffer: PPM1D antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PPM1D antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 μg/ml.)

Related Product Information for anti-PPM1D antibody

Wild-type p53 induced phosphatase 1 (WIP1) / protein phosphatase magnesium-dependent 1 delta (PPM1D), a protein identified in the p53 DNA response pathway, is a member of the PP2C family of serine/threonine protein phosphatases which selectively inactivates p38 MAPK and dephosphorylates the ATM/ATR targets, Chk1 and p53 (1,2). Studies have shown that it is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (2,3). PPM1D deletion results in a reduction of T and B cell function and compromised cell division, rendering cells resistant to becoming cancerous and slowing tumor development (4,5).

NCBI and Uniprot Product Information

• NCBI GI #: 4505997
• NCBI GeneID: 8493
• NCBI Accession #: NP_003611
• NCBI GenBank Nucleotide #: NM_003620.3
• UniProt Accession #: Q8IVR6; Q53XP4; Q6P991; O15297
• Molecular Weight: Predicted: 67 kDa; ; Observed: 68 kDa
• NCBI Official Full Name: protein phosphatase 1D
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1D
• NCBI Official Symbol: PPM1D
• NCBI Official Synonym Symbols: WIP1; PP2C-DELTA
• NCBI Protein Information: protein phosphatase 1D; protein phosphatase Wip1; wild-type p53-induced phosphatase 1; protein phosphatase 2C delta isoform; protein phosphatase 1D magnesium-dependent, delta isoform
• UniProt Protein Name: Protein phosphatase 1D
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1D
• UniProt Synonym Gene Names: WIP1; PP2C-delta
• UniProt Entry Name: PPM1D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]

Uniprot Description

PPM1D: a magnesium-dependent Ser/Thr phosphatase. A p53-induced phosphatase that interacts with the nuclear isoform of uracil DNA glycosylase, UNG2, and suppresses base excision repair. Might contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Cell cycle regulation; EC 3.1.3.16; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: negative regulation of cell proliferation; response to radiation; response to bacterium; G2/M transition of mitotic cell cycle; protein amino acid dephosphorylation

Disease: Breast Cancer