Western Blot (WB)
(PPARGC Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PPARGC antibody detected the PPARGC protein (arrow).)
Rabbit anti-Human PPARGC Polyclonal Antibody | anti-PPARGC antibody
PPARGC (PPARGC1A, LEM6, PGC1, PGC1A, PPARGC1, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6) (MaxLight 490)
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
PPARGC; Polyclonal Antibody; PPARGC (PPARGC1A; LEM6; PGC1; PGC1A; PPARGC1; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; Ligand effect modulator 6) (MaxLight 490); PPARGC1A; Ligand effect modulator 6; anti-PPARGC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-PPARGC antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
PPARGC1A antibody is generated from rabbits immunized with a Gst fusion protein from human PPARGC1A.
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PPARGC Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PPARGC antibody detected the PPARGC protein (arrow).)
Western Blot (WB)
(PPARGC Antibody western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PPARGC antibody detected the PPARGC protein (arrow).)
Flow Cytometry (FC/FACS)
(PPARGC Antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PPARGC Antibody flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PPARGC antibody
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
Product Categories/Family for anti-PPARGC antibody
References
Summermatter, S., et al. J. Biol. Chem. 285(43):32793-32800(2010). Rowe, G.C., et al. Circ. Res. 107(7):825-838(2010). Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010). Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010). Sarzynski, M.A., et al. Int J Obes (Lond) (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
798
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
NCBI Official Symbol
PPARGC1A
NCBI Official Synonym Symbols
LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1(alpha)
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1-alpha; L-PGC-1alpha; PPARGC-1-alpha; ligand effect modulator-6; PPAR gamma coactivator variant form; peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcrip
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Gene Name
PPARGC1A
UniProt Synonym Gene Names
LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha
UniProt Entry Name
PRGC1_HUMAN
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Product Notes
The PPARGC ppargc1a (Catalog #AAA6336165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPARGC (PPARGC1A, LEM6, PGC1, PGC1A, PPARGC1, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6) (MaxLight 490) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPARGC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPARGC ppargc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPARGC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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