Rabbit anti-Human PPARa Polyclonal Antibody | anti-PPARA antibody

PPARa, NT (Peroxisome Proliferator Activated Receptor, alpha)

Cat. Num. AAA648348

• Gene Names: PPARA; PPAR; NR1C1; hPPAR; PPARalpha
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PPARa; Polyclonal Antibody; NT (Peroxisome Proliferator Activated Receptor; alpha); Anti -PPARa; anti-PPARA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes the human PPAR alpha at 52kD. Species sequence homology: mouse, rat, bovine, canine, golden hamster, and boar alpha protein100% and human, chimpanzee and rhesus monkey PPAR 88%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-PPARA antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:8000-1:32,000; Western Blot: 1:500-1:2,000
• Immunogen: Synthetic peptide corresponding to aa1-18, MVDTESPLCPLSPLEAGD, of human PPAR alpha.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PPARA antibody

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPARgamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Product Categories/Family for anti-PPARA antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 50348666
• NCBI GeneID: 5465
• NCBI Accession #: NP_001001928.1
• NCBI GenBank Nucleotide #: NM_001001928.2
• UniProt Accession #: Q07869; Q16241; Q6I9S0; Q92486; Q92689; Q9Y3N1; B0G0X3
• Molecular Weight: 52,225 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor alpha
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor alpha
• NCBI Official Symbol: PPARA
• NCBI Official Synonym Symbols: PPAR; NR1C1; hPPAR; PPARalpha
• NCBI Protein Information: peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3
• UniProt Protein Name: Peroxisome proliferator-activated receptor alpha
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARA
• UniProt Synonym Gene Names: NR1C1; PPAR; PPAR-alpha
• UniProt Entry Name: PPARA_HUMAN

NCBI Description

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety

By similarity. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Ref.1 Ref.13 Ref.15 Ref.16

Subunit structure: Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its transcriptional activity

By similarity. Interacts with NCOA3 and NCOA6 coactivators. Interacts with ASXL1 AND ASXL2. Ref.13 Ref.14 Ref.16 Ref.17 Ref.18 Ref.20

Subcellular location: Nucleus.

Tissue specificity: Skeletal muscle, liver, heart and kidney. Ref.2 Ref.3

Sequence similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain.