Chemiluminescence
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat anti-Human PP2A-B' Polyclonal Antibody | anti-PP2A-B' antibody
PP2A-B' (B56) (protein phosphatase 2A Activator, regulatory subunit 4, PPP2R4 Phosphotyrosyl Phosphatase Activator, PP2A, PP2A, Subunit B', PR53 Isoform, PR53, PTPA, Serine/threonine-protein Phosphatase 2A Regulatory Subunit B')
Gene Names
PP2A-B'; anon-WO0118547.420; B'; B'/PR61; B56-1; BcDNA:GM05554; CG 7913; CG7901; CG7913; dB56-1; DmelCG7913; dPP2A; i234; PP2A; PP2A B'; PP2A-B; wrd
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
PP2A-B'; Polyclonal Antibody; PP2A-B' (B56) (protein phosphatase 2A Activator; regulatory subunit 4; PPP2R4 Phosphotyrosyl Phosphatase Activator; PP2A; Subunit B'; PR53 Isoform; PR53; PTPA; Serine/threonine-protein Phosphatase 2A Regulatory Subunit B'); Anti -PP2A-B' (B56) (protein phosphatase 2A Activator; anti-PP2A-B' antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Ppp2r4/Pp2a. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as liquid in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% BSA.
Applicable Applications for anti-PP2A-B' antibody
Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32,000
Dilution: ELISA: 1:32,000
Immunogen
Synthetic peptide corresponding to C-GSLLPIHPVTSG, from the C-Terminus of human Ppp2r4/Pp2a (NP_821068.1; NP_066954.2; NP_821070.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chemiluminescence
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-PP2A-B' antibody
PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72. The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma. The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
Product Categories/Family for anti-PP2A-B' antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,708 Da
NCBI Official Full Name
PP2A-B', isoform I
NCBI Official Symbol
PP2A-B'
NCBI Official Synonym Symbols
anon-WO0118547.420; B'; B'/PR61; B56-1; BcDNA:GM05554; CG 7913; CG7901; CG7913; dB56-1; DmelCG7913; dPP2A; i234; PP2A; PP2A B'; PP2A-B; wrd
NCBI Protein Information
CG7913 gene product from transcript CG7913-RK; PP2A-B', isoform K; CG7913-PK; PP2A-B'-PK; PP2A B' regulatory subunit; well rounded; regulatory B' subunit of PP2A; protein phosphatase 2A; protein phosphatase 2A-B'; protein phosphatase 2A regulatory B subunit; well-rounded; PP2A B' subunit; PP2A-B', isoform J; CG7913-PJ; PP2A-B'-PJ; PP2A-B', isoform E; CG7913-PE; PP2A-B'-PE; PP2A-B', isoform D; CG7913-PD; PP2A-B'-PD; PP2A-B', isoform I; CG7913-PI; PP2A-B'-PI; PP2A-B', isoform C; CG7913-PC; PP2A-B'-PC; PP2A-B', isoform B; CG7913-PB; PP2A-B'-PB; PP2A-B', isoform A; CG7913-PA; PP2A-B'-PA
UniProt Protein Name
PP2A-B', isoform K
UniProt Gene Name
PP2A-B'
UniProt Entry Name
Q5BIJ2_DROME
Research Articles on PP2A-B'
Similar Products
Product Notes
The PP2A-B' pp2a-b' (Catalog #AAA620501) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The PP2A-B' (B56) (protein phosphatase 2A Activator, regulatory subunit 4, PPP2R4 Phosphotyrosyl Phosphatase Activator, PP2A, PP2A, Subunit B', PR53 Isoform, PR53, PTPA, Serine/threonine-protein Phosphatase 2A Regulatory Subunit B') reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A-B' can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:32,000. Researchers should empirically determine the suitability of the PP2A-B' pp2a-b' for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A-B', Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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