Western Blot (WB)
(Western blot analysis of anti-hPPP2CA/B Antibody (N-term) in A2058 cell line lysates (35ug/lane). PPP2CA/B (arrow) was detected using the purified Pab.)
Rabbit anti-Human PP2A alpha Polyclonal Antibody | anti-PP2A-alpha antibody
PP2A alpha, NT (Serine/Threonine Protein Phosphatase 2A, Catalytic Subunit, alpha Isoform,PP2A-alpha, Replication Protein C, RP-C, PPP2CA) (AP)
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of anti-hPPP2CA/B Antibody (N-term) in A2058 cell line lysates (35ug/lane). PPP2CA/B (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-hPPP2CA/B Antibody (N-term) in A2058 cell line lysates (35ug/lane). PPP2CA/B (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
Uniprot Description
PPP2CA: ubiquitous cytosolic Ser/Thr protein phosphatase, catalytic subunit. The holoenzyme consists of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B'/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: microtubule cytoskeleton; spindle pole; protein phosphatase type 2A complex; membrane; mitochondrion; plasma membrane; nucleus; cytosol; chromosome, pericentric region
Molecular Function: protein dimerization activity; protein C-terminus binding; protein binding; metal ion binding; GABA receptor binding; protein serine/threonine phosphatase activity
Biological Process: mitotic nuclear envelope reassembly; apoptosis; regulation of protein amino acid autophosphorylation; protein amino acid dephosphorylation; response to organic substance; regulation of transcription, DNA-dependent; meiotic cell cycle; regulation of cell differentiation; regulation of growth; mesoderm development; regulation of receptor activity; regulation of DNA replication; inactivation of MAPK activity; second-messenger-mediated signaling; regulation of cell adhesion; fibroblast growth factor receptor signaling pathway; RNA splicing; regulation of Wnt receptor signaling pathway; negative regulation of tyrosine phosphorylation of Stat3 protein; mRNA catabolic process, nonsense-mediated decay; gene expression; regulation of protein catabolic process; mitotic cell cycle; negative regulation of cell growth; ceramide metabolic process
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Product Notes
The PP2A-alpha ppp2ca (Catalog #AAA6495554) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP2A alpha, NT (Serine/Threonine Protein Phosphatase 2A, Catalytic Subunit, alpha Isoform,PP2A-alpha, Replication Protein C, RP-C, PPP2CA) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A alpha can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PP2A-alpha ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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