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Western Blot (WB) (Western blot analysis of MTIF3 expression in HepG2, HUVEC whole cell lysates.)

Rabbit PP15 Polyclonal Antibody | anti-PP15 antibody

PP15 Antibody

Gene Names
NUTF2; NTF2; PP15; NTF-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity chromatography
Synonyms
PP15; Polyclonal Antibody; PP15 Antibody; NTF2; Nuclear transport factor 2; NTF-2; Placental protein 15; anti-PP15 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes endogenous levels of PP15 protein.
Purity/Purification
Immunogen affinity chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/mL (varies by lot)
Sequence Length
127
Applicable Applications for anti-PP15 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PP15.
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of MTIF3 expression in HepG2, HUVEC whole cell lysates.)

Western Blot (WB) (Western blot analysis of MTIF3 expression in HepG2, HUVEC whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of PP15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of PP15 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Product Categories/Family for anti-PP15 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14, 28, 42 kDa
NCBI Official Full Name
nuclear transport factor 2
NCBI Official Synonym Full Names
nuclear transport factor 2
NCBI Official Symbol
NUTF2
NCBI Official Synonym Symbols
NTF2; PP15; NTF-2
NCBI Protein Information
nuclear transport factor 2
UniProt Protein Name
Nuclear transport factor 2
UniProt Gene Name
NUTF2
UniProt Synonym Gene Names
NTF-2; PP151 PublicationManual assertion based on opinion iniRef.1

NCBI Description

This gene encodes a cytosolic factor that facilitates protein transport into the nucleus. The encoded protein is required for nuclear import of the small Ras-like GTPase, Ran which is involved in numerous cellular processes. This protein also interacts with the nuclear pore complex glycoprotein p62. [provided by RefSeq, Apr 2016]

Uniprot Description

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.

Research Articles on PP15

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Product Notes

The PP15 nutf2 (Catalog #AAA9430823) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP15 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PP15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:100. Researchers should empirically determine the suitability of the PP15 nutf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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