Rabbit anti-Human POU3F3 Polyclonal Antibody | anti-POU3F3 antibody

POU3F3, ID (POU3F3, BRN1, OTF8, POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Octamer-binding protein 8, Octamer-binding transcription factor 8) (Azide free) (HRP)

Cat. Num. AAA6336020

• Gene Names: POU3F3; BRN1; OTF8; oct-8; brain-1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POU3F3; Polyclonal Antibody; ID (POU3F3; BRN1; OTF8; POU domain; class 3; transcription factor 3; Brain-specific homeobox/POU domain protein 1; Octamer-binding protein 8; Octamer-binding transcription factor 8) (Azide free) (HRP); Octamer-binding transcription factor 8; anti-POU3F3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-POU3F3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-323 amino acids from the Central region of human POU3F3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POU3F3 Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the POU3F3 antibody detected the POU3F3 protein (arrow).)

Related Product Information for anti-POU3F3 antibody

POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]).[supplied by OMIM].

Product Categories/Family for anti-POU3F3 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006). Kuhlbrodt, K., et al. J. Biol. Chem. 273(26):16050-16057(1998). Sumiyama, K., et al. Mamm. Genome 9(2):180-181(1998). Malik, K.F., et al. Brain Res. Mol. Brain Res. 45(1):99-107(1997). Sumiyama, K., et al. J. Mol. Evol. 43(3):170-178(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 5453936
• NCBI GeneID: 5455
• NCBI Accession #: NP_006227.1
• NCBI GenBank Nucleotide #: NM_006236.2
• UniProt Accession #: P20264; P78379; Q4ZG25
• Molecular Weight: 50,327 Da
• NCBI Official Full Name: POU domain, class 3, transcription factor 3
• NCBI Official Synonym Full Names: POU class 3 homeobox 3
• NCBI Official Symbol: POU3F3
• NCBI Official Synonym Symbols: BRN1; OTF8; oct-8; brain-1
• NCBI Protein Information: POU domain, class 3, transcription factor 3
• UniProt Protein Name: POU domain, class 3, transcription factor 3
• Protein Family: POU domain, class 3, transcription factor
• UniProt Gene Name: POU3F3
• UniProt Synonym Gene Names: BRN1; OTF8; Brain-1; Brn-1; Oct-8; OTF-8
• UniProt Entry Name: PO3F3_HUMAN

NCBI Description

This gene encodes a POU-domain containing protein that functions as a transcription factor. The encoded protein recognizes an octamer sequence in the DNA of target genes. This protein may play a role in development of the nervous system. [provided by RefSeq, Apr 2015]

Uniprot Description

Function: Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3')

By similarity.

Subcellular location: Nucleus.

Tissue specificity: Brain.

Sequence similarities: Belongs to the POU transcription factor family. Class-3 subfamily.Contains 1 homeobox DNA-binding domain.Contains 1 POU-specific domain.