Rabbit POU2F1/OCT1 Polyclonal Antibody | anti-POU2F1/OCT1 antibody

Phospho-POU2F1/OCT1 (Ser385) Antibody

Cat. Num. AAA9614089

• Gene Names: POU2F1; OCT1; OTF1; oct-1B
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: POU2F1/OCT1; Polyclonal Antibody; Phospho-POU2F1/OCT1 (Ser385) Antibody; FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain; class 2; transcription factor 1; POU2F1; Lymphoid restricted immunoglobulin octamer binding protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; NF A2; Oct 2; Oct-2; Octamer binding transcription factor 2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2_HUMAN; POU domain class 2 transcription factor 2; POU2F2; transcription factor 2; anti-POU2F1/OCT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-POU2F1/OCT1 (Ser385) Antibody detects endogenous levels of POU2F1/OCT1 only when phosphorylated at Ser385.; Tissue Specificity: Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-POU2F1/OCT1 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human POU2F1/OCT1 around the phosphorylation site of Ser385.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated by PRKDC.
• Subunit Structure: Interacts with NR3C1, AR and PGR.
• Similarity: Belongs to the POU transcription factor family. Class-2 subfamily.
• Subcellular Location: Cytoplasm. Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of POU2F1/OCT1 (Phospho-Ser385) using Jurkat whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-POU2F1/OCT1 antibody

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.

NCBI and Uniprot Product Information

• NCBI GI #: 311771667
• NCBI GeneID: 5451
• NCBI Accession #: NP_001185712.1
• NCBI GenBank Nucleotide #: NM_001198783.1
• UniProt Accession #: P14859; P09086
• Molecular Weight: 76,472 Da
• NCBI Official Full Name: POU domain, class 2, transcription factor 1 isoform 2
• NCBI Official Synonym Full Names: POU class 2 homeobox 1
• NCBI Official Symbol: POU2F1
• NCBI Official Synonym Symbols: OCT1; OTF1; oct-1B
• NCBI Protein Information: POU domain, class 2, transcription factor 1; NF-A1; OTF-1; oct-1; octamer-binding protein 1; Octamer-binding transcription factor-1; octamer-binding transcription factor 1
• UniProt Protein Name: POU domain, class 2, transcription factor 1
• UniProt Gene Name: POU2F1
• UniProt Synonym Gene Names: OCT1; OTF1; Oct-1; OTF-1
• UniProt Entry Name: PO2F1_HUMAN

NCBI Description

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]

Uniprot Description

Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; olfactory placode formation