Rabbit anti-Human POU2F1 Polyclonal Antibody | anti-POU2F1 antibody

POU2F1, NT (POU2F1, OCT1, OTF1, POU domain, class 2, transcription factor 1, NF-A1, Octamer-binding protein 1, Octamer-binding transcription factor 1) (AP)

Cat. Num. AAA6335983

• Gene Names: POU2F1; OCT1; OTF1; oct-1B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: POU2F1; Polyclonal Antibody; NT (POU2F1; OCT1; OTF1; POU domain; class 2; transcription factor 1; NF-A1; Octamer-binding protein 1; Octamer-binding transcription factor 1) (AP); Octamer-binding transcription factor 1; anti-POU2F1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-POU2F1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: POU2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human POU2F1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(POU2F1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POU2F1 Antibody detected the POU2F1 protein (arrow).)

Related Product Information for anti-POU2F1 antibody

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.

Product Categories/Family for anti-POU2F1 antibody

Antibodies; Transcription Factors

References

Vuzman, D., et al. Biophys. J. 99(4):1202-1211(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Davila, S., et al. Genes Immun. 11(3):232-238(2010). Laurent, B., et al. Blood 115(3):687-695(2010). Becker, M.L., et al. Pharmacogenet. Genomics 20(1):38-44(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 311771665
• NCBI GeneID: 5451
• NCBI Accession #: NP_002688
• NCBI GenBank Nucleotide #: NM_002697
• UniProt Accession #: P14859; Q5TBT7; Q6PK46; Q8NEU9; Q9BPV1; B1AL91; B1AL93; B4E029; J3KP77
• Molecular Weight: 72,082 Da
• NCBI Official Full Name: POU domain, class 2, transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: POU class 2 homeobox 1
• NCBI Official Symbol: POU2F1
• NCBI Official Synonym Symbols: OCT1; OTF1; oct-1B
• NCBI Protein Information: POU domain, class 2, transcription factor 1; NF-A1; OTF-1; Octamer-binding transcription factor-1; oct-1; octamer-binding protein 1; octamer-binding transcription factor 1
• UniProt Protein Name: POU domain, class 2, transcription factor 1
• Protein Family: POU domain, class 2, transcription factor
• UniProt Gene Name: POU2F1
• UniProt Synonym Gene Names: OCT1; OTF1; Oct-1; OTF-1
• UniProt Entry Name: PO2F1_HUMAN

Uniprot Description

Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; olfactory placode formation