Rabbit anti-Human POLS Polyclonal Antibody | anti-PAPD7 antibody

POLS, ID (PAPD7, POLS, TRF4, DNA polymerase sigma, DNA polymerase kappa, LAK-1, PAP-associated domain-containing protein 7, Terminal uridylyltransferase 5, Topoisomerase-related function protein 4-1)

Cat. Num. AAA645603

• Gene Names: PAPD7; LAK1; POLK; POLS; TRF4; LAK-1; TRF41; TRF4-1; TUTASE5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: POLS; Polyclonal Antibody; ID (PAPD7; TRF4; DNA polymerase sigma; DNA polymerase kappa; LAK-1; PAP-associated domain-containing protein 7; Terminal uridylyltransferase 5; Topoisomerase-related function protein 4-1); Anti -POLS; anti-PAPD7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PAPD7 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: POLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human POLS.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POLS Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the POLS antibody detected the POLS protein (arrow).)

Related Product Information for anti-PAPD7 antibody

The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described.

Product Categories/Family for anti-PAPD7 antibody

Antibodies; Abs to Topoisomerases

NCBI and Uniprot Product Information

• NCBI GI #: 219518042
• NCBI GeneID: 11044
• UniProt Accession #: Q5XG87; O43289; Q17RZ1; Q9Y6C1; A8K1E2
• Molecular Weight: 59,874 Da
• NCBI Official Full Name: POLS protein
• NCBI Official Synonym Full Names: PAP associated domain containing 7
• NCBI Official Symbol: PAPD7
• NCBI Official Synonym Symbols: LAK1; POLK; POLS; TRF4; LAK-1; TRF41; TRF4-1; TUTASE5
• NCBI Protein Information: DNA polymerase sigma; TUTase 5; DNA polymerase kappa; terminal uridylyltransferase 5; polymerase (DNA directed) sigma; polymerase (DNA-directed) sigma; PAP-associated domain-containing protein 7; topoisomerase-related function protein 4-1
• UniProt Protein Name: DNA polymerase sigma
• UniProt Gene Name: PAPD7
• UniProt Synonym Gene Names: POLS; TRF4; TUTase 5; TRF4-1
• UniProt Entry Name: PAPD7_HUMAN

NCBI Description

The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

Function: DNA polymerase, probably involved in DNA repair. May play a role in sister chromatid cohesion. Does not play a role in replication-dependent histone mRNA degradation. Ref.7 Ref.9

Catalytic activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactor: Magnesium or manganese

By similarity.

Subcellular location: Nucleus

Probable.

Sequence similarities: Belongs to the DNA polymerase type-B-like family.Contains 1 PAP-associated domain.

Sequence caution: The sequence AAD45198.1 differs from that shown. Reason: Frameshift at position 474.