Rabbit anti-Human POLR2D Polyclonal Antibody | anti-POLR2D antibody

POLR2D, ID (POLR2D, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16kD subunit)

Cat. Num. AAA6002083

• Gene Names: POLR2D; RBP4; RPB16; HSRBP4; HSRPB4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: POLR2D; Polyclonal Antibody; ID (POLR2D; DNA-directed RNA polymerase II subunit RPB4; DNA-directed RNA polymerase II subunit D; RNA polymerase II 16kD subunit); Anti -POLR2D; anti-POLR2D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-POLR2D antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: POLR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-79 amino acids from the Central region of human POLR2D.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(POLR2D Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the POLR2D antibody detected the POLR2D protein (arrow).)

Western Blot (WB)

(Western blot analysis of POLR2D (arrow) using rabbit polyclonal POLR2D Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the POLR2D gene.)

Related Product Information for anti-POLR2D antibody

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

Product Categories/Family for anti-POLR2D antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 14714582
• NCBI GeneID: 5433
• UniProt Accession #: O15514; Q52LT4
• Molecular Weight: 16,311 Da
• NCBI Official Full Name: POLR2D protein
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide D
• NCBI Official Symbol: POLR2D
• NCBI Official Synonym Symbols: RBP4; RPB16; HSRBP4; HSRPB4
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB4; RNA polymerase II subunit B4; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit hsRBP4; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II 16 kDa polypeptide
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB4
• UniProt Gene Name: POLR2D
• UniProt Synonym Gene Names: RNA polymerase II subunit B4; RPB16
• UniProt Entry Name: RPB4_HUMAN

NCBI Description

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. Belongs to the eukaryotic RPB4 RNA polymerase subunit family.

Protein type: RNA processing; Nucleotide Metabolism - purine; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Transferase; Transcription initiation complex

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: single-stranded RNA binding; translation initiation factor binding; DNA-directed RNA polymerase activity; nucleotide binding; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; DNA repair; nuclear mRNA splicing, via spliceosome; positive regulation of translational initiation; mRNA export from nucleus during heat stress; mRNA capping; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; gene expression; mRNA catabolic process, deadenylation-dependent decay