FCM/FACS (Flow Cytometry)
(Separation of human CD1d positive lymphocytes (red-filled) from human CD1d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
CD1d Antibody | anti-CD1d antibody
Anti-Hu CD1d Purified
Gene Names
CD1D; R3; CD1A
Reactivity
Human, Non-human primates
Applications
Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD1d; N/A; Anti-Hu CD1d Purified; CD1d molecule; anti-CD1d antibody
Reactivity
Human, Non-human primates
Isotype
Mouse IgG2b kappa
Clone Number
51.1
Specificity
The mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers.
Form/Format
Purified
Purified by protein-A affinity chromatography.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purified by protein-A affinity chromatography.
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD1d antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
human CD1d
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD1d positive lymphocytes (red-filled) from human CD1d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Separation of human CD1d positive lymphocytes (red-filled) from human CD1d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 ?g/ml, GAM APC).)
Related Product Information for anti-CD1d antibody
CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.
References
*Kim HS, Garcia J, Exley M, Johnson KW, Balk SP, Blumberg RS: Biochemical characterization of CD1d expression in the absence of beta2-microglobulin. J Biol Chem. 1999 Apr 2;274(14):9289-95., URL: https://pubmed.ncbi.nlm.nih.gov/10092605/,*Durante-Mangoni E, Wang RJ, Shaulov A, He Q, Nasser I, Afdhal N, Koziel MJ, Exley MA: Hepatic CD1d expression in hepatitis C virus infection and recognition by resident proinflammatory CD1d-reactive T cells. J Immunol. 2004 Aug 1;173(3):2159-66., URL: https://pubmed.ncbi.nlm.nih.gov/15265953/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
335
NCBI Official Full Name
Antigen-presenting glycoprotein CD1d
NCBI Official Synonym Full Names
CD1d molecule
NCBI Official Symbol
CD1D
NCBI Official Synonym Symbols
R3; CD1A
NCBI Protein Information
antigen-presenting glycoprotein CD1d; R3G1; thymocyte antigen CD1D; CD1D antigen, d polypeptide; T-cell surface glycoprotein CD1d; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D
UniProt Protein Name
Antigen-presenting glycoprotein CD1d
UniProt Gene Name
CD1D
UniProt Entry Name
CD1D_HUMAN
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Product Notes
The CD1d cd1d (Catalog #AAA129285) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD1d Purified reacts with Human, Non-human primates and may cross-react with other species as described in the data sheet. AAA Biotech's CD1d can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD1d cd1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD1d, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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