Rabbit anti-Human PODXL Polyclonal Antibody | anti-PODXL antibody

PODXL, CT (PODXL, PCLP, PCLP1, Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1) (FITC)

Cat. Num. AAA6335491

• Gene Names: PODXL; PC; PCLP; Gp200; PCLP-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PODXL; Polyclonal Antibody; CT (PODXL; PCLP; PCLP1; Podocalyxin; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1) (FITC); Podocalyxin-like protein 1; anti-PODXL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PODXL.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PODXL antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 410-439 in the C-terminal region of human PODXL.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in MDA-MB231 cell line lysates (35ug/lane) usingMBS646256. This demonstrates that MBS646256 detected the PODXL protein (arrow).)

Western Blot (WB)

(Western Blot analysis in Hela whole cell lysate (20ug) using MBS646256 (1:1000). Secondary goat anti-rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 59kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-PODXL antibody

Antibodies; Proteins

References

1. Schopperle, W.M., et al. Biochem. Biophys. Res. Commun. 398(3):372-376(2010). 2. Hsu, Y.H., et al. Am. J. Pathol. 176(6):3050-3061(2010). 3. Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010). 4. Drossopoulou, G.I., et al. Am. J. Physiol. Renal Physiol. 297 (3), F594-F603 (2009). 5. Thomas, S.N., et al. Am. J. Physiol., Cell Physiol. 296 (3), C505-C513 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 33598950
• NCBI GeneID: 5420
• NCBI Accession #: NP_005388.2
• NCBI GenBank Nucleotide #: NM_005397.3
• UniProt Accession #: O00592; Q52LZ7; Q53ER6; A6NHX8
• Molecular Weight: 55,386 Da
• NCBI Official Full Name: podocalyxin isoform 2
• NCBI Official Synonym Full Names: podocalyxin-like
• NCBI Official Symbol: PODXL
• NCBI Official Synonym Symbols: PC; PCLP; Gp200; PCLP-1
• NCBI Protein Information: podocalyxin; GCTM-2 antigen; podocalyxin-like protein 1
• UniProt Protein Name: Podocalyxin
• Protein Family: Podocalyxin
• UniProt Gene Name: PODXL
• UniProt Synonym Gene Names: PCLP; PCLP1; PC; PCLP-1
• UniProt Entry Name: PODXL_HUMAN

Uniprot Description

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: ruffle; extracellular space; lamellipodium; integral to plasma membrane; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; lipid raft; filopodium

Molecular Function: protein binding

Biological Process: cell migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; cell adhesion; positive regulation of cell-cell adhesion mediated by integrin; leukocyte migration; positive regulation of cell migration; regulation of microvillus biogenesis