Rabbit anti-Rat, Mouse Podocin Polyclonal Antibody | anti-NPHS2 antibody

Podocin polyclonal antibody

Cat. Num. AAA3014591

• Gene Names: NPHS2; PDCN; SRN1
• Reactivity: Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Synonyms: Podocin; Polyclonal Antibody; Podocin polyclonal antibody; NPHS2; anti-NPHS2 antibody

• Host: Rabbit
• Reactivity: Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Podocin polyclonal antibody detects endogenous levels of Podocin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-NPHS2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human Podocin.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of Podocin pAb at 1:1000 dilutionLane1:The Kidney tissue lysate of Mouse(20ug) Lane2:The Kidney tissue lysate of Rat(30ug))

Related Product Information for anti-NPHS2 antibody

The pathological conditions of SRN1 correlate well with mutations at the NPHS2 gene, where expression of a protein known as Podocin occurs. Abnormal or inefficient signaling through Podocin protein-dependent networks contributes to the development of podocyte dysfunction and proteinuria. The human NPHS2 gene maps to chromosome 1q25-q31 and encodes a 383 amino acid protein. Podocin is an integral membrane protein that appears to fold into a hairpin-like structure with intracellular amino- and carboxy-termini. Transmembrane and cytoplasmic portions of Podocin share homology to the corresponding regions of the stomatin family proteins. Expression of high-order oligomers of Podocin in glomerular podocytes may reflect a scaffolding function that influences proper function of the glomerular filtration barrier, which is necessary for renal stability.

NCBI and Uniprot Product Information

• NCBI GI #: 7657615
• NCBI GeneID: 7827
• NCBI Accession #: NP_055440.1
• NCBI GenBank Nucleotide #: NM_014625.2
• UniProt Accession #: Q9NP85; Q8N6Q5; B1AM32; B1AM33
• Molecular Weight: 42,201 Da
• NCBI Official Full Name: podocin
• NCBI Official Synonym Full Names: nephrosis 2, idiopathic, steroid-resistant (podocin)
• NCBI Official Symbol: NPHS2
• NCBI Official Synonym Symbols: PDCN; SRN1
• NCBI Protein Information: podocin
• UniProt Protein Name: Podocin
• Protein Family: Podocin
• UniProt Gene Name: NPHS2
• UniProt Entry Name: PODO_HUMAN

NCBI Description

This gene encodes the glomerular protein podocin which plays a role in the regulation of glomerular permeability, and acts as a linker between the plasma membrane and the cytoskeleton. Defects in this gene are the cause of autosomal recessive steroid-resistant nephrotic syndrome (SRN). SRN is characterized by onset between three months and five years, resistance to steroid therapy and rapid progression to end-stage renal disease. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: protein complex; endoplasmic reticulum; integral to plasma membrane; plasma membrane; intercellular junction; lipid raft

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2