Rabbit anti-Human Podocalyxin Polyclonal Antibody

Podocalyxin (Podocalyxin-like protein-1, PC, PCLP, PCLP1, PODXL, MGC138240, GCTM-2 Antigen Gp200)

Cat. Num. AAA613130

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Podocalyxin; Polyclonal Antibody; Podocalyxin (Podocalyxin-like protein-1; PC; PCLP; PCLP1; PODXL; MGC138240; GCTM-2 Antigen Gp200); Anti -Podocalyxin (Podocalyxin-like protein-1; anti-Podocalyxin antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Species Crossreactivity: This antibody reacts with the human protein. Other species have not been determined.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.

• Applicable Applications for anti-Podocalyxin antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: A synthetic peptide within an internal region (residues 350-450) of the human Podocalyxin protein. [Swiss-Prot# O00592]. Homology: 100% with human, 80% with dog, 76% with rat, and 70% with mouse.
• Cellular Localization: Membrane
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Podocalyxin antibody

Podocalyxin is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiologic role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Product Categories/Family for anti-Podocalyxin antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 2213813
• NCBI Official Full Name: podocalyxin-like protein
• Protein Family: Podocalyxin

Uniprot Description

PODXL: Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. Belongs to the podocalyxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7q32-q33

Cellular Component: ruffle; extracellular space; microvillus membrane; integral to plasma membrane; lamellipodium; cytoplasm; apical plasma membrane; plasma membrane; lipid raft; filopodium

Molecular Function: protein binding

Biological Process: cell migration; negative regulation of cell adhesion; negative regulation of cell-cell adhesion; cell adhesion; leukocyte migration; positive regulation of cell-cell adhesion mediated by integrin; regulation of microvillus biogenesis; positive regulation of cell migration