Rabbit anti-Human PNPLA4 Polyclonal Antibody | anti-PNPLA4 antibody

PNPLA4, CT (PNPLA4, DXS1283E, GS2, Patatin-like phospholipase domain-containing protein 4, Protein GS2) (APC)

Cat. Num. AAA6335401

• Gene Names: PNPLA4; GS2; DXS1283E; iPLA2eta
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PNPLA4; Polyclonal Antibody; CT (PNPLA4; DXS1283E; GS2; Patatin-like phospholipase domain-containing protein 4; Protein GS2) (APC); Protein GS2; anti-PNPLA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 253

• Applicable Applications for anti-PNPLA4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PNPLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human PNPLA4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PNPLA4 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PNPLA4 antibody detected the PNPLA4 protein (arrow).)

Immunohistochemistry (IHC)

(PNPLA4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PNPLA4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PNPLA4 antibody

This gene encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y.

Product Categories/Family for anti-PNPLA4 antibody

Antibodies; Enzymes; Phospholipase

References

Gao, J.G., et al. Mol. Genet. Metab. 96(4):253-260(2009). Kienesberger, P.C., et al. J. Lipid Res. 50 SUPPL, S63-S68 (2009): Johansson, L.E., et al. PLoS ONE 4 (4), E5327 (2009): Gao, J.G., et al. Biochem. Biophys. Res. Commun. 360(2):501-506(2007). Wilson, P.A., et al. J. Lipid Res. 47(9):1940-1949(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 42415471
• NCBI GeneID: 8228
• NCBI Accession #: NP_004641.1; NP_001135861.1; NP_001166143.1
• NCBI GenBank Nucleotide #: NP_004641.1
• UniProt Accession #: P41247
• NCBI Official Full Name: patatin-like phospholipase domain-containing protein 4 isoform 1
• NCBI Official Synonym Full Names: patatin like phospholipase domain containing 4
• NCBI Official Symbol: PNPLA4
• NCBI Official Synonym Symbols: GS2; DXS1283E; iPLA2eta
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 4
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 4
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA4
• UniProt Synonym Gene Names: DXS1283E; GS2
• UniProt Entry Name: PLPL4_HUMAN

NCBI Description

This gene encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y. [provided by RefSeq, Feb 2010]