Rabbit anti-Human PNP Polyclonal Antibody | anti-PNP antibody

PNP Antibody (Center)

Cat. Num. AAA9212480

• Gene Names: PNP; NP; PUNP; PRO1837
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PNP; Polyclonal Antibody; PNP Antibody (Center); Purine nucleoside phosphorylase; Inosine phosphorylase; Inosine-guanosine phosphorylase; NP; anti-PNP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PNP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 145-178 amino acids from the Central region of human PNP.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 289

• Applicable Applications for anti-PNP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from Daudi, HT-1080, K562, MCF-7, MDA-MB-231 cell line (from left to right), using PNP Antibody (Center). MBS9212480 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-PNP antibody

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.

Product Categories/Family for anti-PNP antibody

Crown Antibodies; Neuroscience

References

Williams S.R.,et al.Nucleic Acids Res. 12:5779-5787(1984).
Williams S.R.,et al.J. Biol. Chem. 262:2332-2338(1987).
Yu L.,et al.Environ. Health Perspect. 111:1421-1427(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 157168362
• NCBI GeneID: 4860
• NCBI Accession #: NP_000261.2
• NCBI GenBank Nucleotide #: NM_000270.3
• UniProt Accession #: P00491; Q15160; Q5PZ03; B2R8S5; D3DS00
• Molecular Weight: 32118
• NCBI Official Full Name: purine nucleoside phosphorylase
• NCBI Official Synonym Full Names: purine nucleoside phosphorylase
• NCBI Official Symbol: PNP
• NCBI Official Synonym Symbols: NP; PUNP; PRO1837
• NCBI Protein Information: purine nucleoside phosphorylase
• UniProt Protein Name: Purine nucleoside phosphorylase
• Protein Family: Polyribonucleotide nucleotidyltransferase
• UniProt Gene Name: PNP
• UniProt Synonym Gene Names: NP; PNP
• UniProt Entry Name: PNPH_HUMAN

NCBI Description

This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

NP: a metabolic enzyme with purine-nucleoside phosphorylase activity. Defects are the cause of nucleoside phosphorylase deficiency (NP deficiency), with severe T-cell immunodeficiency with neurologic disorder in children.

Protein type: Transferase; EC 2.4.2.1; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: cytoskeleton; cytoplasm; intracellular; cytosol

Molecular Function: purine binding; nucleoside binding; drug binding; phosphate binding; purine-nucleoside phosphorylase activity

Biological Process: response to drug; nucleobase, nucleoside and nucleotide metabolic process; nicotinamide riboside catabolic process; inosine catabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; positive regulation of T cell proliferation; immune response; positive regulation of alpha-beta T cell differentiation; purine salvage; purine base metabolic process

Disease: Purine Nucleoside Phosphorylase Deficiency