Rabbit anti-Human PNLIP Polyclonal Antibody | anti-PNLIP antibody

PNLIP, CT (PNLIP, Pancreatic triacylglycerol lipase) (Azide free) (HRP)

Cat. Num. AAA6335294

• Gene Names: PNLIP; PL; PTL; PNLIPD
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PNLIP; Polyclonal Antibody; CT (PNLIP; Pancreatic triacylglycerol lipase) (Azide free) (HRP); Pancreatic triacylglycerol lipase; anti-PNLIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PNLIP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PNLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-341 amino acids from the C-terminal region of human PNLIP.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PNLIP Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the PNLIP antibody detected the PNLIP protein (arrow).)

Related Product Information for anti-PNLIP antibody

This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas.

Product Categories/Family for anti-PNLIP antibody

Antibodies; Enzymes; Lipase

References

Colin, D.Y., et al. Protein Eng. Des. Sel. 23(5):365-373(2010). Ranaldi, S., et al. Biochemistry 49(10):2140-2149(2010). Bourbon-Freie, A., et al. J. Biol. Chem. 284(21):14157-14164(2009). Ranaldi, S., et al. Biochemistry 48(3):630-638(2009). Colin, D.Y., et al. Biochem. Biophys. Res. Commun. 370(3):394-398(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 10835000
• NCBI GeneID: 5406
• NCBI Accession #: NP_000927.1
• NCBI GenBank Nucleotide #: NM_000936.2
• UniProt Accession #: P16233; Q5VSQ2
• Molecular Weight: 51,157 Da
• NCBI Official Full Name: pancreatic triacylglycerol lipase
• NCBI Official Synonym Full Names: pancreatic lipase
• NCBI Official Symbol: PNLIP
• NCBI Official Synonym Symbols: PL; PTL; PNLIPD
• NCBI Protein Information: pancreatic triacylglycerol lipase; triacylglycerol acylhydrolase
• UniProt Protein Name: Pancreatic triacylglycerol lipase
• Protein Family: Pancreatic triacylglycerol lipase
• UniProt Gene Name: PNLIP
• UniProt Synonym Gene Names: PL; PTL
• UniProt Entry Name: LIPP_HUMAN

NCBI Description

This gene is a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. This gene is expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

PNLIP: a member of the lipase gene family. It encodes a carboxyl esterase that hydrolyzes insoluble, emulsified triglycerides, and is essential for the efficient digestion of dietary fats. expressed specifically in the pancreas. [provided by RefSeq, Jul 2008]

Protein type: EC 3.1.1.3; Lipid Metabolism - glycerolipid; Secreted; Secreted, signal peptide; Hydrolase

Chromosomal Location of Human Ortholog: 10q26.1

Cellular Component: extracellular region

Molecular Function: triacylglycerol lipase activity; metal ion binding

Biological Process: phototransduction, visible light; cholesterol absorption; retinoid metabolic process; lipid catabolic process; lipid digestion

Disease: Pancreatic Lipase Deficiency