Western Blot (WB)
(PNCK Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PNCK antibody detected the PNCK protein (arrow).)
Rabbit anti-Human PNCK Polyclonal Antibody | anti-PNCK antibody
PNCK, CT (PNCK, Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase) (AP)
Gene Names
PNCK; BSTK3; CaMK1b
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PNCK; Polyclonal Antibody; CT (PNCK; Calcium/calmodulin-dependent protein kinase type 1B; CaM kinase I beta; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase) (AP); Pregnancy up-regulated non-ubiquitously-expressed CaM kinase; anti-PNCK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PNCK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the C-terminal region of human PNCK.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PNCK Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PNCK antibody detected the PNCK protein (arrow).)
Western Blot (WB)
(PNCK Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PNCK antibody detected the PNCK protein (arrow).)
Related Product Information for anti-PNCK antibody
PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).
Product Categories/Family for anti-PNCK antibody
References
Deb, T.B., et al. Am. J. Physiol., Cell Physiol. 295 (2), C365-C377 (2008): Gardner, H.P., et al. Cancer Res. 60(19):5571-5577(2000). Gardner, H.P., et al. Genomics 63(2):279-288(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,500 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type 1B isoform a
NCBI Official Synonym Full Names
pregnancy up-regulated non-ubiquitously expressed CaM kinase
NCBI Official Symbol
PNCK
NCBI Official Synonym Symbols
BSTK3; CaMK1b
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type 1B; caMKI-beta; caM-KI beta; caM kinase IB; caM kinase I beta; pregnancy upregulated non-ubiquitously expressed CaM kinase; pregnancy up-regulated non-ubiquitously-expressed CaM kinase
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type 1B
Protein Family
UniProt Gene Name
PNCK
UniProt Synonym Gene Names
CaM kinase IB; CaM-KI beta; CaMKI-beta
UniProt Entry Name
KCC1B_HUMAN
NCBI Description
PNCK is a member of the calcium/calmodulin-dependent protein kinase family of protein serine/threonine kinases (see CAMK1; MIM 604998) (Gardner et al., 2000 [PubMed 10673339]).[supplied by OMIM, Mar 2008]
Uniprot Description
CAMK1B: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; EC 2.7.11.17; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK1 family
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cytoplasm; nucleus
Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding
Biological Process: protein amino acid phosphorylation
Research Articles on PNCK
Similar Products
Product Notes
The PNCK pnck (Catalog #AAA6335235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PNCK, CT (PNCK, Calcium/calmodulin-dependent protein kinase type 1B, CaM kinase I beta, Pregnancy up-regulated non-ubiquitously-expressed CaM kinase) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PNCK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PNCK pnck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PNCK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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