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Western Blot (WB) (Western blotAll lanes: PMM1 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:NIH3T3 whole cell lysateLane 3:293T whole cell lysateLane 4:mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 30kDaObserved band size: 30kDa)

Rabbit anti-Human, mouse PMM1 Polyclonal Antibody | anti-PMM1 antibody

PMM1 Antibody

Gene Names
PMM1; Sec53
Reactivity
Human, mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
PMM1; Polyclonal Antibody; PMM1 Antibody; Phosphomannomutase 1; PMM 1; PMMH-22; PMMH22; anti-PMM1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
262
Applicable Applications for anti-PMM1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
Immunogen
Recombinant human Phosphomannomutase 1 protrin(2-262aa)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-86200 / sc-86201
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: PMM1 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:NIH3T3 whole cell lysateLane 3:293T whole cell lysateLane 4:mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 30kDaObserved band size: 30kDa)

Western Blot (WB) (Western blotAll lanes: PMM1 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:NIH3T3 whole cell lysateLane 3:293T whole cell lysateLane 4:mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 30kDaObserved band size: 30kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas tissue using MBS7001010 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas tissue using MBS7001010 at dilution 1:100)
Related Product Information for anti-PMM1 antibody
Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,747 Da
NCBI Official Full Name
phosphomannomutase 1
NCBI Official Synonym Full Names
phosphomannomutase 1
NCBI Official Symbol
PMM1
NCBI Official Synonym Symbols
Sec53
NCBI Protein Information
phosphomannomutase 1
UniProt Protein Name
Phosphomannomutase 1
UniProt Gene Name
PMM1
UniProt Synonym Gene Names
PMMH22; PMM 1
UniProt Entry Name
PMM1_HUMAN

NCBI Description

Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

PMM1: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain. Belongs to the eukaryotic PMM family.

Protein type: Carbohydrate Metabolism - fructose and mannose; EC 5.4.2.8; Isomerase; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cell soma; cytosol

Molecular Function: metal ion binding; phosphomannomutase activity; protein binding

Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; GDP-mannose biosynthetic process; mannose metabolic process; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine

Research Articles on PMM1

Similar Products

Product Notes

The PMM1 pmm1 (Catalog #AAA7001010) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PMM1 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PMM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the PMM1 pmm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PMM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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