Rabbit PMEPA1 Polyclonal Antibody | anti-PMEPA1 antibody

PMEPA1 Polyclonal Antibody

Cat. Num. AAA3007496

• Gene Names: PMEPA1; STAG1; TMEPAI
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >103% (by SDS-PAGE).
• Synonyms: PMEPA1; Polyclonal Antibody; PMEPA1 Polyclonal Antibody; Protein TMEPAI; Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein; anti-PMEPA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PMEPA1 polyclonal antibody detects endogenous levels of PMEPA1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >103% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 259

• Applicable Applications for anti-PMEPA1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 115-159 of Human PMEPA1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PMEPA1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:The uterus tissue lysate of Mouse (40ug) Lane4:The testis tissue lysate of Rat (40ug))

Related Product Information for anti-PMEPA1 antibody

Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal. Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4.

NCBI and Uniprot Product Information

• NCBI GI #: 364023812
• NCBI GeneID: 56937
• NCBI Accession #: NP_001242905.1
• NCBI GenBank Nucleotide #: NP_001242905.1
• UniProt Accession #: Q969W9
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: protein TMEPAI isoform d
• NCBI Official Synonym Full Names: prostate transmembrane protein, androgen induced 1
• NCBI Official Symbol: PMEPA1
• NCBI Official Synonym Symbols: STAG1; TMEPAI
• NCBI Protein Information: protein TMEPAI
• UniProt Protein Name: Transmembrane prostate androgen-induced protein
• Protein Family: Protein
• UniProt Gene Name: PMEPA1
• UniProt Synonym Gene Names: STAG1; TMEPAI
• UniProt Entry Name: PMEPA_HUMAN

NCBI Description

This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.31-q13.33

Cellular Component: Golgi membrane; early endosome membrane; integral to membrane; plasma membrane; endosome membrane

Molecular Function: protein binding; WW domain binding

Biological Process: transforming growth factor beta receptor signaling pathway; androgen receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway