Rabbit anti-Human, Mouse PLXNC1 Polyclonal Antibody | anti-PLXNC1 antibody

PLXNC1, CT (PLXNC1, VESPR, Plexin-C1, Virus-encoded semaphorin protein receptor, CD232) (PE)

Cat. Num. AAA6335135

• Gene Names: PLXNC1; CD232; VESPR; PLXN-C1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PLXNC1; Polyclonal Antibody; CT (PLXNC1; VESPR; Plexin-C1; Virus-encoded semaphorin protein receptor; CD232) (PE); CD232; anti-PLXNC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLXNC1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PLXNC1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1025-1054 in the C-terminal region of human PLXNC1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6006313. This demonstrates that MBS6006313 detected PLXNC1 (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue using MBS6006313 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6006313 for immunohistochemistry.)

Product Categories/Family for anti-PLXNC1 antibody

Antibodies; CD Markers

References

1. Liu, H., et al. Cell 142(5):749-761(2010). 2. Lazova, R., et al. Am J Dermatopathol 31(2):177-181(2009). 3. Scott, G.A., et al. J. Invest. Dermatol. 129(4):954-963(2009). 4. Scott, G.A., et al. J. Invest. Dermatol. 128(1):151-161(2008). 5. Li, Y., et al. PLoS ONE 3 (7), E2707 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5032223
• NCBI GeneID: 10154
• NCBI Accession #: NP_005752.1
• NCBI GenBank Nucleotide #: NM_005761.2
• UniProt Accession #: O60486; Q59H25
• Molecular Weight: 175,742 Da
• NCBI Official Full Name: plexin-C1
• NCBI Official Synonym Full Names: plexin C1
• NCBI Official Symbol: PLXNC1
• NCBI Official Synonym Symbols: CD232; VESPR; PLXN-C1
• NCBI Protein Information: plexin-C1
• UniProt Protein Name: Plexin-C1
• Protein Family: Plexin
• UniProt Gene Name: PLXNC1
• UniProt Synonym Gene Names: VESPR

NCBI Description

This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8 ().