Western Blot (WB)
(All lanes : Anti-PLK4 Antibody (C-Term) at 1:2000 dilutionLane 1: T47D whole cell lysateLane 2: THP-1 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human PLK4 Polyclonal Antibody | anti-PLK4 antibody
PLK4 Antibody (C-Term)
Western Blot (WB)
(All lanes : Anti-PLK4 Antibody (C-Term) at 1:2000 dilutionLane 1: T47D whole cell lysateLane 2: THP-1 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PLK4 Antibody (C-Term) at 1:2000 dilutionLane 1: T47D whole cell lysateLane 2: THP-1 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Uniprot Description
PLK4: a kinase of the PLK family. Localizes to centrosomes and the cleavage furrow during cytokinesis. Interacts with the protein kinase Tec.
Protein type: Protein kinase, Other; Nucleolus; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.21; Other group; PLK family
Chromosomal Location of Human Ortholog: 4q28
Cellular Component: centriole; centrosome; nucleolus; cytosol; cleavage furrow
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: organelle organization and biogenesis; positive regulation of centriole replication; centriole replication; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation
Disease: Microcephaly And Chorioretinopathy, Autosomal Recessive, 2
Research Articles on PLK4
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Product Notes
The PLK4 plk4 (Catalog #AAA9216360) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLK4 Antibody (C-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the PLK4 plk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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