Western Blot (WB)
(Western blot analysis of extracts of MCF-7 cells, using PLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5min.)
Rabbit PLK2 Polyclonal Antibody | anti-PLK2 antibody
PLK2
IHC: 1:100-1:200
Western Blot (WB)
(Western blot analysis of extracts of MCF-7 cells, using PLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5min.)
Western Blot (WB)
(Western blot analysis of extracts of MCF-7 cells, using PLK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5min.)
NCBI and Uniprot Product Information
Calculated MW: 78kDa
NCBI Description
The protein encoded by this gene is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. This gene is most abundantly expressed in testis, spleen and fetal tissues, and its expression is inducible by serum, suggesting that it may also play an important role in cells undergoing rapid cell division. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
PLK2: a kinase of the PLK family. Participates in a mitotic checkpoint in which p53-dependent activation Plk2 prevents mitotic catastrophe following spindle damage. Plk2(-/-) embryos show retarded growth and skeletal development late in gestation. Apparently plays a role for Plk2 in the cell cycle.
Protein type: Tumor suppressor; Protein kinase, Other; Kinase, protein; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Other group; PLK family
Chromosomal Location of Human Ortholog: 5q12.1-q13.2
Cellular Component: centriole; centrosome; dendrite; intracellular
Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; protein complex binding; ATP binding
Biological Process: regulation of centriole replication; positive regulation of I-kappaB kinase/NF-kappaB cascade; mitotic spindle organization and biogenesis; positive regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; Ras protein signal transduction; regulation of synaptic plasticity; protein amino acid phosphorylation; memory; Rap protein signal transduction; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Research Articles on PLK2
Similar Products
Product Notes
The PLK2 plk2 (Catalog #AAA9127715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PLK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:100-1:200. Researchers should empirically determine the suitability of the PLK2 plk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
